Dynamic Change of Secondary Metabolites and spectrum-effect relationship of Malus halliana Koehne flowers during blooming

摘要

Malus halliana Koehne flowers have been used as a Chinese traditional medicine to treat metrorrhagia. In this study, the dynamic changes in its secondary metabolites and spectrum-effect relationship of inhibition on a-glucosidase during blooming were investigated. The changes in the contents of three flavonoids (phloretin-4'-O-glycosidase, afzeloside, and 3-hydroxyphloridzin) were determined by high performance liquid chromatography (HPLC) and changes in inhibitory effect on a-glucosidase were evaluated in vitro. Then, spectrum-effect relationship was evaluated by partial least square method. The results indicated that the contents of three flavonoids and inhibition of a-glucosidase activity in vitro showed a fluctuating downward trend, thereinto, the maximum contents of phloretin-4'-0-glycosidase, afzeloside, and 3-hydroxyphloridzin reached 157.43±0.36, 17.27±0.06 and 22.67±0.35 (mg/g), respectively. In spectrum-effect relationship assay, matched 40 mutual peaks, thereinto, P2, P5, P6-P_(12), P_(14) (3-hydroxyphloridzin), P_(16)-P_(19), P_(20_ (phloretin-4'-0-glycosidase), P_(24), P_(26), P_(29), P_(31), P_(33), P_(34), P_(36) P_(39) and P_(40) were positively correlated to inhibitory effect on a-glucosidase in vitro. P1, P3, P4, P_(13), P_(15), P_(21)-P_(23), P_(27), P_(28), P_(30) (afzeloside), P_(32), P_(35), P_(37) and P_(38) were negatively related to inhibitory effect on a-glucosidase in vitro.