F-19-MRI for monitoring human NK cells in vivo

摘要

The availability of clinical-grade cytokines and artificial antigen-presenting cells has accelerated interest in using natural killer (NK) cells as adoptive cellular therapy (ACT) for cancer. One of the technological shortcomings of translating therapies from animal models to clinical application is the inability to effectively and non-invasively track these cells after infusion in patients. We have optimized the nonradioactive isotope fluorine-19 (F-19) as a means to label and track NK cells in preclinical models using magnetic resonance imaging (MRI). Human NK cells were expanded with interleukin (IL)-2 and labeled in vitro with increasing concentrations of F-19. Doses as low as 2 mg/mL F-19 were detected by MRI. NK cell viability was only decreased at 8 mg/mL F-19. No effects on NK cell cytotoxicity against K562 leukemia cells were observed with 2, 4 or 8 mg/mL F-19. Higher doses of F-19, 4 mg/mL and 8 mg/mL, led to an improved F-19 signal by MRI with 3 x 10(11) F-19 atoms per NK cell. The 4 mg/mL F-19 labeling had no effect on NK cell function via secretion of granzyme B or interferon gamma (IFN gamma), compared to NK cells exposed to vehicle alone. F-19-labeled NK cells were detectable immediately by MRI after intratumoral injection in NSG mice and up to day 8. When F-19-labeled NK cells were injected subcutaneously, we observed a loss of signal through time at the site of injection suggesting NK cell migration to distant organs. The F-19 perfluorocarbon is a safe and effective reagent for monitoring the persistence and trafficking of NK cell infusions in vivo, and may have potential for developing novel imaging techniques to monitor ACT for cancer.