Experimental Study on Biochemical Markers of Mandibular Bone Tissue Metabolism in Rats following the Implantation of PAW-1 and Geistlich Bio-OSS Collagen

摘要

The study is based on the analysis of experimental results and determination of the bone metabolism markers by histochemical and biochemical methods performed on animals. The animals were implanted biovitroceramics PAW-1 in the mandible in the form of spongy matrix composed of fluorhydroxyapatite and microcrystallized wollastonite in a glass matrix, elaborated by the company PONETI (Romania) and Geistlich Bio-Oss-Collagen in the form of block (a mixture of granules, 0.25-1mm in size) and 10% fibers of porcine collagen. Geistlich Bio-Oss Collagen is a natural mineral bone of bovine origin, comparable both chemically and structurally to the mineralized human bone. It has a spongy consistency that allows simple modeling, and collagen facilitates the adaptation of the material on the defect site. The biochemical blood analysis showed an increased activity of bone thermolabile alkaline phosphatase over 30 days, the maximum being reached over 60 days + 20% (p< 0.05) from the beginning of the experiment. The activity of tartrate-resistant bone acid phosphatase, on the contrary, decreased veridically by 22% over 60 days after implantation. Over 30 days after the beginning of the experiment under the action of the implanted material, the level of average molecules (AM) increased by 35% (p < 0.05), while nucleotide substances (NS) increased by 51% (p < 0.05). Within 60 days after implantation, the values of these indices showed a decreasing tendency. The fact mentioned above denotes that their osteoregenerative action increases under the influence of implanted materials.