Effects of oridonin on immune cells, Th1/Th2 balance and the expression of BLys in the spleens of broiler chickens challenged with ce:italic>Salmonella pullorum/ce:italic>

摘要

The aim of this study was to investigate the effects of oridonin (ORI) on the immune cells, Th1/Th2balance and the expression of B lymphocyte stimulator (BLys) in the spleens of broilers infected withSalmonella pullorum. In a completely randomized design, 300 one-day-old AA male broilers were divided to 5 treatments. The groups included a noninfection control (CON) group receiveed a basal diet; aS. pulloruminfect control group received the basal diet; andS. pulloruminfect group receiveed the basal diet plus 50, 80, and 100?mg/kg ORI, respectively. The results showed thatSalmonellachallenge increased the relative weights of the spleen, white blood cell counts, lymphocyte and heterophil percentage, H/L ratio, the concentration and mRNA levels of spleen proinflammatory mediators, including tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), IL-4, IL-6, and IL-10, as well as the anti-inflammatory target Blys (P??.05), and modulated the Th1/Th2 balance (P??.05). ORI pretreatment decreased the relative weight of the spleen and inhibited the release and expression of these proinflammatory mediators and the anti-inflammatory target BLys. The results suggested that ORI supplementation may have immunosuppressive and multiple modulation effects on activated microglia through modulation of the Th1/Th2 balance and BLys expression.