Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data‐independent acquisition strategy

Gethings Lee A.; Richardson Keith; Wildgoose Jason; Lennon Sarah; Jarvis Sheba; Bevan Charlotte L.; Vissers Johannes P.C.; Langridge James I.

摘要

Rationale >A novel data‐independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time‐of‐flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches. </section> <section xml:id="rcm7941-sec-0002" numbered="no"> <title type="main">Methods >Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented. </section> <section xml:id="rcm7941-sec-0003" numbered="no"> <title type="main">Results >The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data. </section> <section xml:id="rcm7941-sec-0004" numbered="no"> <title type="main">Conclusions >In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification. </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”> <section xml：id =“rcm7941-sec-0001”编号=“no”> <title type =“main “>理由</标题> >详细描述了一种新的数据独立的采集方法，其结合在正交加速飞行时间（TOF）质量分析仪前面的扫描四轮轴。描述了这种方法，并将属性进行比较并与其他DIA方法对比。</ p> </段> <部分XML：ID =“RCM7941-SEC-0002”编号为“否”> <标题类型=“main” >方法[/标题> >对靶向和未确定的脂质素识别策略的特异性施用，来自霰弹枪和LC分离的脂质族学实验的数据。</ p> </ section> <section XML：ID =“RCM7941-SEC-0003”编号=“否”> <标题类型=“main”>结果</ title> >突出显示了快速四极扫描技术的好处，包括复杂速度和特异性的改进提供高质量的定性和定量数据的混合物。</ p> </ section> <secto xml：id =“rcm7941-sec-0004”编号=“否”> <标题类型=“main”>结论</ title> < P>特别是扫描四极杆提供的高特异性改善了脂质鉴定的定性信息。</ p> </段> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26627/'>《Rapid Communications in Mass Spectrometry: RCM》</a> <b style="margin: 0 2px;">|</b><span>2017年第19期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gethings Lee A.&option=202" target="_blank" rel="nofollow">Gethings Lee A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Richardson Keith&option=202" target="_blank" rel="nofollow">Richardson Keith;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wildgoose Jason&option=202" target="_blank" rel="nofollow">Wildgoose Jason;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lennon Sarah&option=202" target="_blank" rel="nofollow">Lennon Sarah;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jarvis Sheba&option=202" target="_blank" rel="nofollow">Jarvis Sheba;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bevan Charlotte L.&option=202" target="_blank" rel="nofollow">Bevan Charlotte L.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vissers Johannes P.C.&option=202" target="_blank" rel="nofollow">Vissers Johannes P.C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Langridge James I.&option=202" target="_blank" rel="nofollow">Langridge James I.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Waters CorporationStamford Avenue Wilmslow UK;</p> <p>Waters CorporationStamford Avenue Wilmslow UK;</p> <p>Waters CorporationStamford Avenue Wilmslow UK;</p> <p>Waters CorporationStamford Avenue Wilmslow UK;</p> <p>Department of Surgery and CancerImperial CollegeLondon UK;</p> <p>Department of Surgery and CancerImperial CollegeLondon UK;</p> <p>Waters CorporationStamford Avenue Wilmslow UK;</p> <p>Waters CorporationStamford Avenue Wilmslow UK;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 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<b>[P]</b> . <span> 外国专利：  JP5562933B2 </span> <span> . 2014-07-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：包括执行该程序和该处理质谱数据的方法的计算机程序和该计算机程序的计算机程序产品是具有包括傅立叶的液相色谱质谱仪无效周期信号的质谱数据的处理方法。包括执行数据处理权宜之计的变换质谱仪 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130501128985.html">CHROMATOGRAPHY–MASS SPECTROMETRY DATA PROCESSING METHOD, CHROMATOGRAPHY–MASS SPECTROMETRY DEVICE, AND CHROMATOGRAPHY–MASS SPECTROMETRY DATA PROCESSING PROGRAM</a> <b>[P]</b> . <span> 外国专利：  WO2021240710A1 </span> <span> . 2021-12-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：色谱 - 质谱数据处理方法，色谱 - 质谱装置和色谱 - 质谱数据处理程序 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130499897856.html">RAPID ANALYSIS AND IDENTIFICATION OF LIPIDS FROM LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS) DATA</a> <b>[P]</b> . <span> 外国专利：  EP3665476A4 </span> <span> . 2021-05-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：液相色谱 - 质谱（LC-MS）数据的快速分析和鉴定脂质 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704024020374','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div 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