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首页> 外文期刊>Luminescence: The journal of biological and chemical luminescence >Expression of genes encoding the luciferase from Photobacterium leiognathiPhotobacterium leiognathi in Escherichia coli Escherichia coliEscherichia coli Rosetta (DE3) and its application in NADH detection
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Expression of genes encoding the luciferase from Photobacterium leiognathiPhotobacterium leiognathi in Escherichia coli Escherichia coliEscherichia coli Rosetta (DE3) and its application in NADH detection

机译：将荧光素酶的基因的表达从光纤维酸杆菌Leioghathi 在 大肠杆菌中的Photobacterium Leiognathi 大肠杆菌大肠杆菌罗萨（DE3） 及其在NADH检测中的应用

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Abstract > Cloning of genes encoding the luciferase from Photobacterium leiognathi YL in <fc> <fi>Escherichia coli</fi> </fc> Rosetta (DE3) was performed successfully and the expressed forms of lux AB were purified to homogeneity. Experimental measurements revealed that luciferase from Photobacterium leiognathi YL has good thermal stability and a high residual activity at extreme pH values, which are extremely important for its various ecological, industrial and medical applications. Furthermore, we made a first attempt for quantitative detection of NADH by recombinant <fc> <fi>E. coli</fi> </fc> Rosetta (DE3) coupled enzyme system. A good linear relationship between luminescence intensity and NADH with low (1–12?nmol/L) and high (10–500?nmol/L) concentration was observed, whose standard curve was y ?=?772.97 × ?+?4041.1, R 2 ?=?0.9884 and y ?=?1710 × ?+?4.99?×?10 5 , R 2 ?=?0.9727, respectively. Our results demonstrate a high sensitivity of recombinant <fc> <fi>E. coli</fi> </fc> coupled enzyme system to NADH on the basis of high soluble expression of recombinant luciferase and continuous and stable expression of some NAD(P)H‐dependent flavin mononucleotide (FMN) reductases. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >从光纤维酶的基因克隆到 Photobacterium leioghathi </ i> Yl FC> <FI>大肠杆菌（Rosetta（DE3）成功进行，表达形式的勒克斯ab </ i>被纯化为均匀性。实验测量显示，来自光杆菌的荧光素酶Leioghathi </ i> Y1具有良好的热稳定性和极高pH值的高残留活性，这对于其各种生态，工业和医疗应用来说是非常重要的。此外，我们首先尝试通过重组<Fc> <Fi> e进行NADH的定量检测。 COLI </ FI> </ FC> Rosetta（DE3）偶联酶系统。观察到发光强度和NADH的发光强度和NADH之间的良好线性关系，其标准曲线是</ i>？=？ 772.97 ×</ i>？4041.1， R </ i> 2 </ sup>？=？0.9884和 Y </ i>？=？1710 ×4.99？×10 5 </ sup>， r </ i> 2 </ sup>？=？0.9727。我们的结果表明了重组<Fc> <Fi> E的高灵敏度。基于重组荧光素酶的高可溶性表达和一些NAD（P）H依赖性黄酮单核苷酸（FMN）还原酶的高可溶性表达，COLI </ FI> </ FC> NADH偶联酶系统。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26441/'>《Luminescence: The journal of biological and chemical luminescence》</a> <b style="margin: 0 2px;">|</b><span>2018年第6期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xuan Guanhua&option=202" target="_blank" rel="nofollow">Xuan Guanhua;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiao Qilin&option=202" target="_blank" rel="nofollow">Xiao Qilin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Jingxue&option=202" target="_blank" rel="nofollow">Wang Jingxue;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Hong&option=202" target="_blank" rel="nofollow">Lin Hong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pavase Tushar&option=202" target="_blank" rel="nofollow">Pavase Tushar;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Food Safety Laboratory College of Food Science and EngineeringOcean University of ChinaQingdao P. R. China;</p> <p>Food Safety Laboratory College of Food Science and EngineeringOcean University of ChinaQingdao P. R. China;</p> <p>Food Safety Laboratory College of Food Science and EngineeringOcean University of ChinaQingdao P. R. China;</p> <p>Food Safety Laboratory College of Food Science and EngineeringOcean University of ChinaQingdao P. R. China;</p> <p>Food Safety Laboratory College of Food Science and EngineeringOcean University of ChinaQingdao P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1345.html" title="生物光学">生物光学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bacterial luciferase&option=203" rel="nofollow">bacterial luciferase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lux AB gene&option=203" rel="nofollow">lux AB gene;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=molecular cloning and expression&option=203" rel="nofollow">molecular cloning and expression;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=NADH detection&option=203" rel="nofollow">NADH detection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=RT‐PCR&option=203" rel="nofollow">RT‐PCR;</a> </p> <div class="translation"> 机译：细菌荧光素酶;LUX AB基因;分子克隆和表达;NADH检测;RT-PCR; </div> </li> <li> <span class="lefttit">入库时间</span> <span>2022-08-20 04:53:50</span> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023986052.html">Expression of genes encoding the luciferase from <i >Photobacterium leiognathiPhotobacterium leiognathi in <fc > <fi>Escherichia coli</fi> </fc> <fi >Escherichia coli</fi>Escherichia coli Rosetta (DE3) and its application in NADH detection</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xuan Guanhua&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xuan Guanhua,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiao Qilin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xiao Qilin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Jingxue&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang Jingxue,</a> <a href="/journal-foreign-26441/" target="_blank" rel="nofollow" class="tuijian_authcolor">Luminescence: The journal of biological and chemical luminescence .</a> <span>2018</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：将荧光素酶的基因的表达从光纤维酸杆菌Leioghathi </ i>在<fc> <fi>大肠杆菌中的Photobacterium Leiognathi </ fi> </ fi> <fi>大肠杆菌</ fi>大肠杆菌罗萨（DE3） 及其在NADH检测中的应用</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022031847.html">Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative <fc > <fi>Escherichia coli</fi> </fc> <fi >Escherichia coli</fi>Escherichia coli</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ellis Samuel J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ellis Samuel J.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yasir Muhammad&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yasir Muhammad,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Browning Douglas F.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Browning Douglas F.,</a> <a href="/journal-foreign-15891/" target="_blank" rel="nofollow" class="tuijian_authcolor">Cellular microbiology .</a> <span>2019</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：氧气和接触人肠上皮，独立地刺激肠溶中的毒力基因表达<Fc> <fi>大肠杆菌</ Fi> </ Fi> <Fi>大肠杆菌的大肠杆菌（Escherichia Coli）</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025419534.html">Selective and sensitive <fc > <fi>Escherichia coli</fi> </fc> <fi >Escherichia coli</fi>Escherichia coli detection based on a T4 bacteriophage‐immobilized multimode microfiber</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Yanpeng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Li Yanpeng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ma Hui&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ma Hui,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gan Lin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gan Lin,</a> <a href="/journal-foreign-33894/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Biophotonics .</a> <span>2018</span><span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：选择性和敏感 <FC> <fi>大肠杆菌</ fi> </ FC> <fi>大肠杆菌</ fi>大肠杆菌 基于T4噬菌体固定的多模超细纤维检测</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-290291_thesis/0705018317170.html">Co-expression of Recombinant Human Epidermal Growth Factor (rhEGF) In Escherichia coli BL21 (DE3) with Bacillus cereus Phospholipase C</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=A. 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Wang Bin</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王斌&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王斌</a> <span> <a href="/conference-cn-323/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十一届中国智能交通年会 </a> <span> <span> . 2016</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020312384332.html">水稻FIS类多梳蛋白基因启动子特性及基因表达分析</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡雷&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 胡雷</a> <span> . 2010</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120678945.html">一个在大肠杆菌中组成型表达的启动子及其在大肠杆菌中的应用</a> <b>[P]</b> . <span> 中国专利： CN101560516B </span> <span> . 2010.12.08</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120105755674.html">一个在大肠杆菌中组成型表达的启动子及其在大肠杆菌中的应用</a> <b>[P]</b> . <span> 中国专利： CN101560516A </span> <span> . 2009-10-21</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130400497340.html">GENE-THERAPEUTIC DNA-VECTOR BASED ON GENE-THERAPEUTIC DNA-VECTOR VTVAF17, CARRYING TARGET GENE SELECTED FROM GROUP OF GENES ANG, ANGPT1, VEGFA, FGF1, HIF1Α, HGF, SDF1, KLK4, PDGFC, PROK1, PROK2 TO INCREASE EXPRESSION LEVEL OF SAID TARGET GENES, METHOD FOR PRODUCTION AND USE THEREOF, STRAIN ESCHERICHIA COLI SCS110-AF/VTVAF17-ANG, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-ANGPT1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-VEGFA, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-FGF1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-HIF1Α, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-HGF, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-SDF1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-KLK4, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-PDGFC, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-PROK1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-PROK2, CARRYING GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, METHOD FOR INDUSTRIAL PRODUCTION OF GENE-THERAPEUTIC DNA VECTOR</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2730664C2 </span> <span> . 2020-08-24</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于基因治疗DNA载体VTVAF17的基因治疗DNA载体，携带选自基因ANG，ANGPT1，VEGFA，FGF1，HIF1α，HGF，SDF1，KLK4，PDGFC，PROK1，PROK2表达的靶基因所述的靶标基因，其生产和使用方法，应变大肠埃希氏菌SCS110-AF / VTVAF17-ANG或大肠埃希氏菌SCS110-AF / VTVAF17-ANGPT1或大肠埃希氏菌SCS110-AF / VTVAF17-VEGFA或大肠埃希氏菌SCS110-AF / VTVAF17-FGF1，或大肠埃希氏菌SCS110-AF / VTVAF17-HIF1A，或大肠埃希氏菌COLI SCS110-AF / VTVAF17-HGF，或大肠埃希氏菌SCS110-AF / VTVAF17-SDF1，或大肠埃希氏菌SCS110-AF / VTVAF17-KLK4，大肠埃希氏菌SCS110-AF / VTVAF17-PDGFC或大肠埃希氏菌SCS110-AF / VTVAF17-PROK2，携带基因-治疗性DNA载体，生产方法，工业生产方法-治疗性DNA载体 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130400537005.html">Gene therapeutic DNA vector based on VTvaf17 gene therapeutic DNA vector carrying a target gene selected from the group of genes ANG, ANGPT1, VEGFA, FGF1, HIF1α, HGF, SDF1, KLK4, PDGFC, PROK1, PROK2 to increase the expression level of these target genes, method its preparation and use, Escherichia coli strain SCS110-AF / VTvaf17-ANG or Escherichia coli SCS110-AF / VTvaf17-ANGPT1 or Escherichia coli SCS110-AF / VTvaf17-VEGFA or Escherichia coli SCS110-AF / VTvaf17-Fichia-SC110 AF / VTvaf17-HIF1α or Escherichia coli SCS110-AF / VTvaf17-HGF or Escherichia coli SCS110-AF / VTvaf17-SDF1 or Escherichia coli SCS110-AF / VTvaf17-KLK4 or Escherichia coli SCS110-AF / VTviFi10 SCF110 AF / VTvaf17-PROK1 or Escherichia coli SCS110-AF / VTvaf17-PROK2 carrying a gene therapy DNA vector, a method for its preparation, a method for the industrial production of a gene therapy DNA vector</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2018147085A </span> <span> . 2020-06-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于VTvaf17基因治疗DNA载体的基因治疗DNA载体，其携带选自ANG，ANGPT1，VEGFA，FGF1，HIF1α，HGF，SDF1，KLK4，PDGFC，PROK1，PROK2的靶基因以增加这些靶标的表达水平基因，方法的制备和使用，大肠杆菌菌株SCS110-AF / VTvaf17-ANG或大肠杆菌SCS110-AF / VTvaf17-ANGPT1或大肠杆菌SCS110-AF / VTvaf17-VEGFA或大肠杆菌SCS110-AF / VTvaf17-Fichia-SC110 AF /VTvaf17-HIF1α或大肠杆菌SCS110-AF / VTvaf17-HGF或大肠杆菌SCS110-AF / VTvaf17-SDF1或大肠杆菌SCS110-AF / VTvaf17-KLK4或大肠杆菌SCS110-AF / VTviFi10 S1携带基因治疗DNA载体的大肠杆菌SCS110-AF / VTvaf17-PROK2，其制备方法，工业生产基因治疗DNA载体的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130400493335.html">GENE-THERAPEUTIC DNA VECTOR BASED ON THE GENE-THERAPEUTIC DNA VECTOR GDTT1_8NAS12, CARRYING THE TARGET GENE SELECTED FROM A GROUP OF GENES DDC, IL10, IL13, IFNB1, TNFRSF4, TNFSF10, BCL2, HGF, IL2 TO INCREASE THE EXPRESSION LEVEL OF SAID TARGET GENES, A METHOD FOR PRODUCTION AND USE THEREOF, A STRAIN ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-DDC OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IL10 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IL13 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IFNB1 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-TNFRSF4 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-TNFSF10 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-BCL2 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-HGF OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IL2, CARRYING A GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, A METHOD FOR INDUSTRIAL PRODUCTION OF A GENE-THERAPEUTIC DNA VECTOR</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2734726C1 </span> <span> . 2020-10-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于基因治疗DNA矢量GDTT1_8NAS12的基因治疗DNA矢量，携带从一组基因中选择的目标基因DDC，IL10，IL13，IFNB1，TNFRSF4，TNFSF10，BCL2，HGF，IL2可以增加表达水平基因，一种生产和使用其的方法，应变大肠埃希氏菌JM110-NAS / GDTT1_8NAS12-DDC或大肠埃希氏菌JM110-NAS / GDTT1_8NAS12-IL10或大肠埃希氏菌JM110-NAS / GDTT1_8NAS12-IL13或大肠埃希氏菌COLI JM110-NAS / GDTT1_8NAS12-IL13 IFNB1或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12-TNFRSF4或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12-TNFSF10或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12-BCL2或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12 IL2，携带基因治疗性DNA载体，其生产方法，工业生产基因治疗性DNA载体的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" 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