DNA extraction of urinary bladder swabs collected from carbonized and decomposing corpses: Possible application in disaster victim identification

摘要

A disaster is an unexpected event causing death or injury to many people. In such events, a large number of casualties may take place, exposing corpses to a harsh environment for days or months. DNA profiling is recognized as one of the primary methods for identifying mass disaster victims, especially when it involves decomposed or fragmented bodies. The objective of this study was to standardize the use of urinary bladder swabs as a source of DNA for the identification of decomposing and carbonized human bodies by Forensic Genetic techniques. Samples' DNA was extracted using both organic and Chelex (R) resin methods; quantified by qPCR and amplified with PowerPlex (R) Fusion System (Promega Corporation). The results of this study show that between the two methodologies used for DNA extraction, the organic method presented higher DNA yields in relation to the minimum acceptable for the amplification, while Chelex (R), although not having a high yield, still allowed obtaining significant amounts of DNA for amplification. The use of bladder swabs has proven to be a viable source of DNA for human identification, since besides reproducible and reliable results, this type of sample allows a significant reduction in the time and cost required for analysis.