Concentration-dependent protein adsorption at the nano-bio interfaces of polymeric nanoparticles and serum proteins

摘要

Aim: A comprehensive understanding of nanoparticle (NP)-protein interaction (protein corona formation) is required. So far, many factors influencing this interaction have been investigated, like size and zeta potential. However, NPs exposure concentration has always been ignored. Herein, we aim to disclose the correlation of NPs exposure concentration with protein adsorption. Materials & methods: Four polymeric NPs systems possessing similar sizes (230 +/- 20 nm) but varied zeta potentials (-30 similar to+ 40 mv) were prepared. Physicochemical properties and protein adsorption upon NP-protein interaction were characterized. Results: Protein adsorption capacity and adsorbed protein types were NPs concentration-dependent. Conclusion: Considering the critical impacts of protein adsorption on NPs delivery, our work could be an urgent warning about the possible risks of dosage adjustment of nanoformulations.