Implantable composite devices of unsintered hydroxyapatite and poly-L-lactide with dispersive marbling morphology to enhance in vivo bioactivity and bioresorbability

摘要

A bone fixation device made of unsintered hydroxyapatite (u-HA) particles uniformly dispersed in a poly-L-lactide matrix and reinforced by compressive forging (uniformly dispersed composite; UDC) has been clinically applied in several fields. However, it has reported some foreign body reactions over a long implantation period due to its slow bioresorbability. To further enhance its bioresorbability, we developed devices comprising a fibrous assembly of poly-L-lactide only three-dimensionally intertwined with particulate hydroxyapatite/poly-L-lactide composite. The biological behavior of the proposed material, provisionally referred to as complementarily reinforced composite (CRC), was compared with that of UDC in vivo. Cylindrical rods of UDC and CRC with 3.2-mm diameters were inserted bilaterally into the intramedullary distal femurs of 32 male Japanese white rabbits. Eight rabbits were euthanized at four, eight, 12, and 25 weeks after insertion. There were no significant differences between the mechanical properties of UDC and CRC over time. However, the results from histological analyses, surface characterization, radiological analyses, and push-out mechanical testing substantiated CRC's superior affinity to bone and enhanced bioactivity and bioresorbability in comparison with UDC. These characteristics were attributed to the dispersive marbling morphology produced by the CRC material's successive connectivity of u-HA particles throughout the PLLA matrix, which would accelerate PLLA hydrolysis degradation by H2O intrusion and enhance the bioactivity of u-HA particles exposed on the interface soon after implantation.