Sinusoidal voltage electrodeposition of PEDOT-Prussian blue nanoparticles composite and its application to amperometric sensing of H2O2 in human blood

摘要

A selective electrochemical sensor based on poly(3,4-ethylenedioxythiophene) (PEDOT) - Prussian blue nano-particles (PBNPs) for hydrogen peroxide (H2O2) determination was prepared by innovative sinusoidal voltage (SV) method. The successful incorporation of citrate-stabilized PBNPs into PEDOT matrix was confirmed by energy dispersive X-ray analysis (EDX), Raman spectroscopy, UV-Vis spectroelectrochemistry and cyclic voltammetry measurements. The SV preparation method provides a PEDOT-PBNPs coating with rough surface morphology and good electrocatalytic activity toward H2O2 reduction. The amperometric response of PEDOT-PBNPs-based sensor at -50 mV vs. Ag/AgCI is linear within the range of concentrations from 5 mu M to 1 mM H2O2 with a detection limit of 1.4 mu M H2O2. The proposed Pt/PEDOT-PBNPs sensor displays good repeatability, reproducibility, operational stability as well as good selectivity toward H2O2 determination in the presence of interfering species like dopamine (DA), uric acid (UA), KNO2 glucose (Glu), KNO3 and ascorbic acid (AA), and was successfully applied to H2O2 determination in human blood samples without biofouling.