Anti-ageing properties of Khelma Longevity (TM): treatment of human fibroblasts increases proteasome levels and decreases the levels of oxidized proteins

摘要

We have determined the putative anti-ageing properties of Khelma Longevity (TM), a formula based on various natural compounds from the Mediterranean area. Human primary fibroblast cultures were treated with a wide range of concentrations of Khelma Longevity (TM) for 1 day or 3 consecutive days. Following these treatments, two major and complementary biomarkers of ageing were measured, namely, the proteasome and the amount of oxidized proteins. It was observed that 24 h of treatment with Khelma Longevity (TM) resulted in a maximum increase of about 41% of the total protein levels of 20S proteasome. Levels of oxidized proteins were reduced by almost 6.5-fold following longer treatments. Specifically we have observed a maximum decrease of protein carbonyls to 84.7% in comparison with nontreated control cells following 3 days of continuous treatment with Khelma Longevity (TM). These results support the notion that formulas rich in natural compounds from the Mediterranean area possess anti-ageing properties. (C) 2017 Elsevier B.V. All rights reserved.