Identification of Genetic Markers Distinguishing Golden Flounders from Normal Olive Flounders Paralichthys olivaceus Using Microsatellite Markers

摘要

Despite its economic importance, olive flounder Paralichthys olivaceus aquaculture industry is facing a crisis with a continuous production decline. There have been many solutions to overcome the complicate predicament proposed. Increasing genetic diversity and discovering new commercial value through selective breeding are among them. The aims of the present study are to increase the selection power of the golden flounders. We examined the genetic diversity of the breeder population of golden flounders and developed selective markers for the golden flounder population. The 6 microsatellite (MS) markers were selected from melanogenesis-related genes, which are believed to be involved in the pigmentation of fish. All markers were polymorphic (exceptP04) and 5 of them had PIC value of 0.6 or above. All makers had distinctive alleles indicating either normal or golden individuals. For examples, from P04 marker, the frequency of an allele (316) in the golden population was 100% and in normal population was 0% (P<0.001). Although some more studies with more samples at the later generations should be performed to confirm this result, the 316 allele from P04 marker could be a distinctive tool for decision of the colors in olive flounders at an early stage of the life cycle.