Evaluation of the genotoxic properties of nickel oxide nanoparticles in vitro and in vivo

摘要

Nickel-based nanoparticles (NPs) are new products with an increasing number of industrial applications that were developed in recent years. NiO NPs are present in several nanotechnological industrial products, and the characterization of their genotoxic potential is essential. The present study assessed the genotoxicity of NiO NPs in vivo and in vitro using the somatic mutation and recombination test in somatic cells of Drosophila melanogaster (SMART), the cytokinesis - block micronucleus assay (CBMN), and the comet assay in a V79 cell line. The NiO NPs used in this study were about 30 nm in mean size. Larvae of Drosophila melanogaster were exposed to 5 mL of five different concentrations (1.31, 2.62, 5.25, 10.5, and 21 mg/mL) of NiO NPs. In turn, V79 cells were treated with a concentration range of 15-2000 mu g/mL NiO NPs. The SMART showed that all concentrations of NiO NPs are genotoxic to the standart (ST) cross when compared to the negative control. On the other hand, only the highest concentration (21 mg/mL) was genotoxic to the HB cross. Somatic recombination was the preferential mechanism lesions were induced in D. melanogaster. The results show that NiO NPs were mutagenic to V79 cells as assessed by the CBMN assay. Significant differences in the frequencies of micronuclei (MN) were observed using the highest NiO NP concentrations (250 and 500 mu g/mL) in the 4- and 24-h treatments, but when 125 mu g/mL NiO NPs was used, such difference was observed only in the 4-h exposure time. The comet assay revealed that 62, 125, 250 and 500 mu g/mL NiO NPs induced a significant increase in DNA damage. The results observed in this study indicate that NiO NPs are genotoxic and mutagenic in vitro and in vivo.