Comparison of the Inhibitory Activities of 5,6-Dihydroergosterol Glycoside - and -Anomers on Skin Inflammation

摘要

Chronic skin inflammatory diseases, such as atopic dermatitis, are associated with a dysfunctional skin barrier due to an increase in various inflammatory stimuli, for instance inflammatory cytokines and chemokines. In particular, CCL17 and CCL22 expression is increased in patients with chronic skin inflammation. In this study, we synthesized several - and -anomers of dihydroergosterol (DHE)-glycosides and assessed their effects on CCL17 and CCL22 expression. We confirmed that the -anomers of DHE-glycosides were superior to -anomers of DHE-glycosides in inhibiting CCL17 and CCL22 mRNA and protein expression. In addition, we determined that DHE-glycoside -anomers showed strong inhibitory activity towards pro-inflammatory cytokine mRNA and protein expression, including that of TNF-, IL-6, and IL-1- in stimulated HaCaT cells. These results imply that DHE-glycoside - and -anomers should be separated during synthesis of drugs for chronic skin inflammation. Our results also suggest that -anomers of DHE-glycosides may play an important role as new drugs for chronic skin inflammation because of their ability to inhibit the skin inflammatory biomarker proteins CCL17 and CCL22.