Study of laser-induced plant fluorescence lifetime imaging technology for plant remote sensing monitor

摘要

Plant fluorescence is too susceptible to interference and unsuitable for plant remote sensing monitor. A plant fluorescence lifetime imaging technology based on plant remote sensing monitor is presented to solve this problem. The technology can be used to analyse plant physiological information for environmental surveillance. The beam expander system was used to expand laser beam at a certain specific wavelengths, and the living plant chlorophyll fluorescence can be excited by this laser beam. Many of the same plant fluorescence signals were excited by continuous laser pulses, and furthermore the start delay time of Intensification Charge Coupled Device was adjusted consecutively in time resolved measurement method. Finally, a complete set of plant continuous fluorescence image was collected by Intensification Charge Coupled Device. The deconvolution can be used to retrieve the high-precision plant fluorescence lifetime of single pixel point in plant continuous fluorescence image. If the plant fluorescence lifetime values from all of pixel points were retrieved, the distribution map of plant fluorescence lifetime can be drawn. The fluorescence lifetime properties of living plants have already been studied preliminarily. The study indicates that the distribution map of plant fluorescence lifetime can more effectively reflect plant chlorophyll content than fluorescence intensity dose. The plant fluorescence lifetime was related to its chlorophyll content in a certain range, and there is a complex and subtle relationship between plant fluorescence lifetime and its physiological information.