Variation in Immune-Related microRNAs Profile in Human Milk Amongst Lactating Women

摘要

Background: Human Milk (HM) is a biological fluid representing the first nutrient fornewborns. It directly impacts the development of the infant's immune system. In this concern, specificmicroRNAs (miRNAs) such as hsa-miR-21, hsa-miR-181a, hsa-miR-150 and hsa-miR-223 are knownto be involved in the innate and acquired immune response.Objective: Herein, these miRNAs were evaluated in frozen and pasteurized samples of human colostrumand HM in order to elucidate the distribution and the expression profile of these biological mediatorsin both biological fluids.Methods: Using quantitative approach qRT-PCR, we analyzed immune-related microRNAs in both,colostrum and HM.Results: Our study provided evidence of a comparable profile of immune specific miRNAs in colostrumand HM. Although we detected all the four miRNAs tested, we point out the prevalence of hsamiR-181a and hsa-miR-223 indicative to act on T and granulocytes cell populations as selective targets.Therefore, these biomolecules could affect newborn’s immune homeostasis at early stages of life.While, variation in immune-related miRNAs was found in HM amongst lactating women, it was notevidenced in colostrum. Of interest, pasteurization procedure did not alter the distribution or the expressionprofile of the miRNAs tested in both colostrum and HM. Herein, we also proposed a simplemethod to determine the quantity of these biomolecules in biological fluids.Conclusion: Considering, this evidence the variation in immune-related miRNAs should be take intoaccount and could be relevant for preterm and hospitalized infants who usually received pasteurizedHM from donors.