Complete genome sequence of Pelolinea submarina MO-CFX1(T) within the phylum Chloroflexi, isolated from subseafloor sediment

摘要

Pelolinea submarina strain MO-CFX1(T) (=JCM 17238(T), =KCTC 5975(T)) is an anaerobic chemoorganotrophic bacterium isolated from subseafloor sediments collected off the Shimokita Peninsula of Japan, north-western Pacific Ocean. This strain was the first isolate belonging to the phylum Chloroflexi from deep-sea sedimentary environment. Here, we report the complete genome sequence of this strain. This genome comprises 3,519,887 bp in a circular chromosome with GC content of 50.6% and contains 3131 protein-coding sequences. The genome contains a complete set of genes for the Embden-Meyerhof-Pamas pathway for glycolysis, coupled with its chemoorganotrophic growth ability with sugars. Similar to previously reported genomes of other members of the phylum Chloroflexi, the genome of this strain also lacks members of protein families associated with lipopolysaccharide production and diderm-specific outer membrane assembly, which are generally observed in typical Gram-negative bacterial cell envelopes. This finding suggests that the present strain possesses a monoderm cell envelope. The obtained genomic data provides insight into the physiological potential and the cell envelope characteristics of P. submarina strain MO-CFX1(T) and help to fill the gap in our knowledge about the phylum Chloroflexi.