A General Strategy for Extrusion Bioprinting of Bio‐Macromolecular Bioinks through Alginate‐Templated Dual‐Stage Crosslinking

Zhu Kai; Chen Nan; Liu Xiao; Mu Xuan; Zhang Weijia; Wang Chunsheng; Zhang Yu Shrike

摘要

Abstract >The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts. The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting. For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free‐standing structures and the maintenance of architectural integrity following bioprinting. This requirement, however, has greatly limited the selection of bioinks, especially for those naturally derived due to their commonly low mechanical properties. Here the generalization of a mechanism for extrusion bioprinting of bio‐macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported. Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio‐macromolecular component and a polysaccharide alginate. The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio‐macromolecular component, alginate can be selectively removed to leave only the desired bio‐macromolecule. It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio‐macromolecular bioinks. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >最近开发的3D生物化工艺技术大大提高了在结构上产生的生物摩擦组织的能力与他们的人类同行相关。选择合适的生物材料作为生物链条是朝向成功生物印刷的关键步骤。例如，生物链的粘度是确定防立式结构的沉积的灵活性以及在生物印刷之后的施加完整性的灵活性。然而，这一要求极大地限制了生物链的选择，特别是对于由于其通常低的机械性能而自然衍生的那些。这里，据报道了挤出生物高分子成分的挤出机理的概括，主要关注胶原蛋白及其衍生物，包括明胶和明胶甲基丙烯酰基甲基酰基甲基酰基。具体地，使用包含所需的生物高分子组分和多糖藻酸盐的复合生物链来采用模板策略。物理可交联的藻酸盐组分用作时间结构支撑，以稳定生物印刷期间构建体的形状;在后续化学或生物高分子组分的物理交联时，可以选择性地除去藻酸盐以仅留下所需的生物大分子。预计这一策略是一般的，可以随时扩大用于使用各种其他生物高分子生物结合。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-24307/'>《Macromolecular bioscience》</a> <b style="margin: 0 2px;">|</b><span>2018年第9期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Kai&option=202" target="_blank" rel="nofollow">Zhu Kai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Nan&option=202" target="_blank" rel="nofollow">Chen Nan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Xiao&option=202" target="_blank" rel="nofollow">Liu Xiao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mu Xuan&option=202" target="_blank" rel="nofollow">Mu Xuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Weijia&option=202" target="_blank" rel="nofollow">Zhang Weijia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Chunsheng&option=202" target="_blank" rel="nofollow">Wang Chunsheng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yu Shrike&option=202" target="_blank" rel="nofollow">Zhang Yu Shrike;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Cardiac SurgeryFudan UniversityShanghai 200032 P. R. China;</p> <p>Department of Cardiac SurgeryFudan UniversityShanghai 200032 P. R. China;</p> <p>Beijing Advanced Innovation Center for Biomedical EngineeringBeihang UniversityBeijing 100083 P. R. China;</p> <p>Division of Engineering in MedicineHarvard Medical SchoolCambridge MA 02139 USA;</p> <p>Department of Chemistry and Institute of Biomedical ScienceFudan UniversityShanghai 200433 P. R. China;</p> <p>Department of Cardiac SurgeryFudan UniversityShanghai 200032 P. R. China;</p> <p>Division of Engineering in MedicineHarvard Medical SchoolCambridge MA 02139 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1185.html" title="高分子化学（高聚物）">高分子化学（高聚物）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alginate&option=203" rel="nofollow">alginate;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioink&option=203" rel="nofollow">bioink;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bio‐macromolecule&option=203" rel="nofollow">bio‐macromolecule;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=extrusion bioprinting&option=203" rel="nofollow">extrusion bioprinting;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microfluidic bioprinting&option=203" rel="nofollow">microfluidic bioprinting;</a> </p> <div class="translation"> 机译：藻酸盐;生物链;生物宏观分子;挤出生物印刷品;微流体生物印刷; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023543196.html">A General Strategy for Extrusion Bioprinting of Bio‐Macromolecular Bioinks through Alginate‐Templated Dual‐Stage Crosslinking</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Kai&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhu Kai,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Nan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chen Nan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Xiao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liu Xiao,</a> <a href="/journal-foreign-24307/" target="_blank" rel="nofollow" class="tuijian_authcolor">Macromolecular bioscience .</a> <span>2018</span><span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过藻酸盐模板双阶段交联挤出生物高分子生殖器的挤出生物监测的一般策略</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025374014.html">Reversible physical crosslinking strategy with optimal temperature for 3D bioprinting of human chondrocyte-laden gelatin methacryloyl bioink</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gu Yawei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gu Yawei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Lei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Lei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Du Xiaoyu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Du Xiaoyu,</a> <a href="/journal-foreign-33717/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of biomaterials applications .</a> <span>2018</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有最佳温度的可逆物理交联策略，用于3D生物印刷甲基丙烯酰基甲基丙烯酰基甲基丙烯酰基</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022388059.html">Bio-inspired hydrogel composed of hyaluronic acid and alginate as a potential bioink for 3D bioprinting of articular cartilage engineering constructs</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-17668/" target="_blank" rel="nofollow" class="tuijian_authcolor">Acta biomaterialia .</a> <span>2020</span><span>,第期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：生物启发水凝胶由透明质酸和藻酸盐作为关节软骨工程构建构建的3D生物印刷的潜在生物蛋白。</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-260462_thesis/0705011530148.html">Photocurable Alginate Bioink Development for Cartilage Replacement Bioprinting</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=H. Mishbak&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">H. Mishbak,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Enes Aslan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Enes Aslan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Glen Cooper&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Glen Cooper,</a> <a href="/conference-foreign-260462/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Conference on Progress in Digital and Physical Manufacturing .</a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于软骨替代品BioPlinting的光固化藻类生物型开发</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005248305.html">A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kai Zhu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kai Zhu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nan Chen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nan Chen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Prof. Xiao Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Prof. Xiao Liu,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过藻酸盐模板双阶段交联的生物大分子生物墨水挤压生物打印的一般策略。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000130673094.html">A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kai Zhu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kai Zhu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nan Chen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nan Chen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiao Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xiao Liu,</a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过藻酸盐模板双阶段交联挤出生物高分子生殖器的挤出生物监测生物监测的一般策略</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_fishery-modernization_thesis/0201298065838.html">采用反应挤出法从掌状昆布中提取藻酸盐</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-7209/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 渔业现代化 </a> </span> <span> . 2009</span><span>,第1期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_rubber-plastics-resources-utilization_thesis/0201272835148.html">双复合挤出机挤出参数的计算</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-9268/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 橡塑资源利用 </a> </span> <span> . 2018</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_brick-tile_thesis/0201213328877.html">双挤出型双级真空挤出机</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李波&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李波</a> <span> <a href="/journal-cn-9673/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 砖瓦 </a> </span> <span> . 2008</span><span>,第009期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_rubber-plastics-energy-saving-environmental-protection_thesis/0201272194619.html">ZE BASIC 62双螺杆挤出机让生物塑料产能直接翻番</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-19117/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 橡塑智造与节能环保 </a> </span> <span> . 2018</span><span>,第7)期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_plastic-development_thesis/0201229289792.html">光—生物双降解聚丙烯挤出片材工艺探讨</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘玉萍&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘玉萍</a> <span> <a href="/journal-cn-26615/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 塑料开发 </a> </span> <span> . 1998</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-4292_thesis/020221028212.html">“锥形同向双螺杆挤出机”技术在高分子聚乙烯双壁波纹管挤出领域的应用前景</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=吴汉民&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 吴汉民</a> <span> <a href="/conference-cn-4292/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2013中国塑协专家委员会年会暨塑料新材料、新技术、新成果交流会 </a> <span> <span> . 2013</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315787106.html">基于32位MCU的三层共挤出交联电缆生产线的智能控制系统的研究与实现</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡科幻&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 胡科幻</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120106462796.html">用兼养和富氮-缺氮两阶段培养策略提高产油微藻生物量和油脂累积的方法</a> <b>[P]</b> . <span> 中国专利： CN102268377A </span> <span> . 2011-12-07</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120107069244.html">用于在用作交联剂的多磷酸盐存在下反应性挤出淀粉质材料的方法、所得产物及其用途</a> <b>[P]</b> . <span> 中国专利： CN104507971A </span> <span> . 2015-04-08</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130499569180.html">NANOCELLULOSE-CONTAINING BIOINKS FOR 3D BIOPRINTING, METHODS OF MAKING AND USING THE SAME, AND 3D BIOSTRUCTURES OBTAINED THEREFROM</a> <b>[P]</b> . <span> 外国专利：  US2021069378A1 </span> <span> . 2021-03-11</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于3D生物监测的含纳米纤维素的生物蛋白，制造和使用相同的方法，以及由此获得的3D生物结构 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130400976683.html">Nanocellulose-containing bioinks for 3D bioprinting, methods of making and using the same, and 3D biostructures obtained therefrom</a> <b>[P]</b> . <span> 外国专利：  AU2019230193A1 </span> <span> . 2020-10-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于3D生物打印的含纳米纤维素的生物墨水，其制备和使用方法以及由此获得的3D生物结构 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130401180208.html">NANOCELLULOSE-CONTAINING BIOINKS FOR 3D BIOPRINTING, METHODS OF MAKING AND USING THE SAME, AND 3D BIOSTRUCTURES OBTAINED THEREFROM</a> <b>[P]</b> . <span> 外国专利：  SG11202008159XA </span> <span> . 2020-09-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于3D生物印刷的含纳米生物素墨水，其制造和使用方法以及从其获得的3D生物结构 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023543196','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704023543196down" data-source="7," data-out-id="P6v5RoKSAQ6fY33zaP3Shzlq+MBlgit+ZHbV3Y7PfrSjKu2b9Yj9EvjtiUDbCuLJ," data-f-source-id="7" data-title="A General Strategy for Extrusion Bioprinting of Bio‐Macromolecular Bioinks through Alginate‐Templated Dual‐Stage Crosslinking" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="0704023543196" id="docid"> <input type="hidden" value="24307" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="A General Strategy for Extrusion Bioprinting of Bio‐Macromolecular Bioinks through Alginate‐Templated Dual‐Stage Crosslinking"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/page.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/reference.js?v=5.8.0"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.8.0"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>