Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp (Carassius auratus gibelio)

摘要

The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD(65), GAD(67), and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P < 0.05 orP < 0.01). While the expressions of GAD(65)and GAD(67)in the kidney at 12 h had strikingly upregulated to 13.81 +/- 1.06** and 150.67 +/- 12.85** times. Treated with the lethal dose of 50% (LD50, 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD(65), GAD(67), and GABA-T in all tissues were increased significantly (P < 0.01). The results of AVM group showed that the mRNA expressions of GAD(65), GAD(67), and GABA-T both in the central and peripheral tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC50, 0.039 mg/L), except for the mRNA inhibitions of GAD(65), GAD(67), and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 +/- 0.02(Delta Delta) x 10(-2), 0.57 +/- 0.06(Delta Delta) x 10(-1)and 0.44 +/- 0.02(Delta Delta) x 10(-1), respectively (P < 0.01).