Genomic structure and characterization of growth hormone receptors from golden pompano Trachinotus ovatus and their expression regulation by feed types

摘要

In this study, sequence analysis showed that ToGHR1 and ToGHR2 encoded polypeptides of 577 and 588 amino acids, respectively. Bioinformatics analysis showed that both ToGHR1 and ToGHR2 contain FN3 domains and transmembrane domains, which have glycosylation and phosphorylation sites. The exons of ToGHR1 and exons 4-10 of ToGHR2 are homologous to exons 2 and 4-9 in Homo sapiens genes, respectively. Only 3 SSR sites in ToGHR1 have SSR polymorphisms, and ToGHR2 has no SSR polymorphisms. ToGHR1 and ToGHR2 have high homology with GHR1 and GHR2 of many fish by BLAST. qRT-PCR was used to examine the expression profile of ToGHR mRNA in 12 normal liver and intestine tissue samples from 3 feed-type groups. The results showed that ToGHR is expressed in all 12 tissues, especially liver and muscle tissues, which showed higher ToGHR expression than that in other tissues (p 0.05). Experiments on feed-type groups may indicate that high levels of LC-PUFA in squid bait can promote ToGHR1 expression and simultaneously inhibit ToGHR2 expression in the liver tissue. In addition, the high levels of LC-PUFA in food could inhibit intestinal ToGHR1 expression, and the intermediate levels may promote intestinal ToGHR1 expression. However, the unsaturated fatty acid content in the food does not affect the expression of intestinal ToGHR2.