Combination of Vortex-Assisted Liquid-Liquid Extraction and Air-Assisted Liquid-Liquid Microextraction for the Extraction of Bisphenol A and Bisphenol B in Canned Doogh Samples

摘要

A sensitive, rapid, and efficient analytical method based on vortex-assisted liquid-liquid extraction combined with air-assisted liquid-liquid microextraction using high performance liquid chromatography-variable wavelength detection has been developed for the extraction and determination of bisphenol A and bisphenol B in canned doogh samples. In this method, the analytes are partitioned into n-hexane during liquid-liquid extraction and then they are enriched using the following air-assisted liquid-liquid microextraction procedure in a few microliters of an ammonia solution used as an extraction solvent. Under the optimum conditions, limits of detection and quantification were 0.82 and 0.54 and 2.7 and 1.5ngmL(-1), for bisphenol A and bisphenol B, respectively. The extraction recoveries and enrichment factors of the selected analytes were 86 and 81% and 4300 and 4050, respectively. The relative standard deviations were lower than 9% for intra- (n=6) and inter-day (n=4) precisions at a concentration of 5ngmL(-1) of each analyte. Finally, some canned doogh samples were effectively analyzed by the proposed method.