Simultaneous Determination of Seven Polycyclic Aromatic Hydrocarbons in Coffee Samples Using Effective Microwave-Assisted Extraction and Microextraction Method Followed by Gas Chromatography-Mass Spectrometry and Method Optimization Using Central Composite Design

摘要

In this research, for the first attempt, we successfully determined seven polycyclic aromatic hydrocarbons (PAHs) in various coffee samples using microwave-assisted extraction and dispersive liquid-liquid microextraction (MAE-DLLME) coupled with gas chromatography-mass spectrometry (GC-MS). The effects of most important variables in microextraction step were investigated and optimized using response surface methodology (RSM) based on central composite design. The calibration curves were linear in the range of 1-200 ng g(-1), with a correlation coefficient (R-2) higher than 0.989. Limits of detection were obtained between 0.1 and 0.3 ng g(-1). The relative standard deviations (RSD%) for seven repeated analysis were less than 8% for all PAH compounds at a concentration of 10 ng g(-1). Relative recoveries were obtained 88.1-101.3%. The satisfactory results obtained by the proposed method and the comparison of these results with previous methods demonstrated that the MAE-DLLME-GC-MS is an accurate, rapid, and reliable sample-pretreatment method with low consumption of the organic solvent.