Optimization Strategy for Extraction of Active Polyphenols from Leaves of Eugenia uniflora Linn

摘要

This study aimed to evaluate the performance of polyphenolic extraction from leaves of Eugenia uniflora by using solvent systems and different extractive conditions. The solvent systems containing water, methanol, ethanol, and acetone (binary, ternary, or quaternary mixtures) were used to produce extracts by turbo-extraction in accordance with a simplex centroid. The extraction performances were determined by dry residue, total tannin, and flavonoid contents, which were used to calculate the extractive efficiency, and content of markers gallic acid, ellagic acid, and myricitrin. Fingerprints were also obtained by high-performance liquid chromatography and high-performance thin layer chromatography. The mixture of H2O to MeOH to EtOH to acetone (46:13:18:23) demonstrate a larger extractive efficiency and was used as solvent to evaluate the influences of drug amount and extraction method. A 3(2)-design with response surface methodology allowed to optimize the extraction conditions, showing better extraction at 5% (w/v) of the herbal drug and by turbo-extraction, standardized condition for extracts from Eugenia uniflora leaves.