Increasing the Survival Rate of Manipulated Cells: Gentle Single Cell Manipulation

摘要

The investigation of living cells is of high relevance in the biosciences. To observe intracellular structures of living cells, specialized tools in combination with fluorescent probes can be used, which grant access to their complex machinery. A possible strategy to investigate these intracellular structures of single living cells is to combine specialized tools with functionalized fluorescent probes. These tools overcome the plasma membrane of a living cell by penetration with pipettes or cantilevers to deliver the fluorescent probes. But these tools affect the survival of a cell significantly.