Two-decade long fertilization induced changes in subsurface soil organic carbon stock vary with indigenous site characteristics

摘要

Soil organic carbon (SOC) sequestration at subsurface layers (i.e. & 20 cm) remains unclear under long-term fertilization practices. Based on long-term datasets of fertilization experiments in four typical Chinese croplands, representing soils with high fertility at Gongzhuling (GZL, black soil) and Chongqing (CQ, purple soil), and low fertility at Zhengzhou (ZZ, aquatic Chao soil) and Qiyang (QY, red soil), we calculated SOC storage, its change relative to initial condition (Delta SOC) in 0-20,20-40 and 40-60 cm. We also obtained annual organic C inputs (OCI; stubble, roots and manure amendment) and derived soil C sequestration efficiency (CSE: the ratio of Delta SOC over OCI) in 0-20 cm and 0-60 cm. The fertilization treatments include cropping with no fertilization (CK), chemical nitrogen, phosphorus and potassium fertilizers (NPK) and combined chemical fertilizers and manure (NPKM). Results showed SOC stock significantly increased with fertilizations (i.e. initial, CK & NPK & NPKM). Relative to initial condition, surface (0-20 cm) and subsurface (20-60 cm) SOC stocks significantly decreased under CK at all sites except GZL, a site with elevated SOC stocks under all fertilizations and depths. Subsurface SOC stocks significantly increased at high fertility soils (i.e., GZL and CQ) but remained no change or significantly decreased at low fertility soils (i.e., ZZ and QY) under NPK and NPKM. Accordingly, CSE derived in 0-60 cm was consistently higher than that in 0-20 cm in high fertility soils but lower in low fertility soils. These results demonstrated that subsurface soils (20-60 cm) remained as C sinks in indigenously high fertility sites but experienced substantial C depletions in low fertility sites. This study informed the need to account for subsurface soil carbon changes for accurate estimates of soil C sequestration capacity under long-term fertilization.