首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes
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Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes

机译:不同TCM综合征初级痛风患者外周血单核细胞中Caspase-1基因转录变体mRNA的表达

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摘要

A large number of studies have shown that cysteinyl aspartate specific protease-1 (CASP1) played an important role in the inflammatory response of primary gout, but the decreased expression of different CASP1 transcript variant could inhibit the activation of IL-1 beta. Our study mainly analyzed the expression level and function of CASP1 gene transcript variant mRNA in peripheral blood mononuclear cells of patients with gout in different TCM syndromes. The expression of CASP1 gene transcript variant and IL-1 beta mRNA in PBMCs were detected in patients with PG [acute phase (AP: 44 cases); nonacute phase (NAP: 52 cases)] and healthy controls (HC: 30 cases) by reverse transcription-polymerase chain reaction and/or real-time quantitative polymerase chain reaction. The expressions of plasma IL-1 beta in patients with PG and HC were detected by enzyme-linked immunosorbent assay. Dysregulated expression of the CASP1 gene and its transcript variant, plasma proinflammatory cytokines in all patients with primary gout in different TCM syndromes, correlation analysis showed that there was negative correlation between the expression of CASP1-gamma gene transcript variant mRNA and IL-1 beta protein in APPG group. The study suggested that CASP1 gene and its transcript variant may play a critical role in the inflammatory response of patients with PG in different phases and TCM syndromes.
机译:大量的研究表明,Cysteinyl天冬氨酸特异性蛋白酶-1(Casp1)在原发性痛风的炎症反应中起重要作用,但不同Casp1转录变体的表达降低可以抑制IL-1β的激活。我们的研究主要分析了不同TCM综合征痛风患者外周血单核细胞中Casp1基因转录物变体mRNA的表达水平和功能。在PG患者中检测到PBMC中CasP1基因转录变体和IL-1βmRNA的表达[急性期(AP:44例);通过逆转录 - 聚合酶链反应和/或实时定量聚合酶链反应,非易于相(NAP:52例)]和健康对照(HC:30例)。通过酶联免疫吸附测定检测PG和HC患者血浆IL-1β的表达。在不同的中医综合征中所有患者中,血浆1基因及其转录物变异的血浆促炎细胞因子的表达表达,相关性分析表明,CasP1-Gamma Gene转录物变体mRNA和IL-1β蛋白的表达之间存在负相关性在APPG组中。该研究表明,CASP1基因及其转录物变异可能在PG患者的不同阶段和中医综合征患者的炎症反应中起重要作用。

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    Chengdu Univ Tradit Chinese Med Sch Clin Med Chengdu 610075 Peoples R China;

    North Sichuan Med Coll Dept Nephrol Affiliated Hosp Nanchong 637000 Peoples R China;

    North Sichuan Med Coll Inst Rheumatol &

    Immunol Affiliated Hosp Nanchong 637000 Peoples R China;

    North Sichuan Med Coll Inst Rheumatol &

    Immunol Affiliated Hosp Nanchong 637000 Peoples R China;

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  • 正文语种 eng
  • 中图分类 临床医学;
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