Effect of Qingxin Kaiqiao Fang on Hippocampus mRNA Expression of the Inflammation-Related Genes IL-1 beta, GFAP, and A beta in an Alzheimer's Disease Rat Model

摘要

Objective. To investigate the effects of QKF on expression of amyloid-beta (A beta), interleukin-1 beta (IL-1 beta), and glial fibrillary acidic protein (GFAP) using a rat model of AD. Materials and Methods. Fifty-six male Sprague-Dawley rats were randomly divided into seven groups (eight rats each): control group, sham-operated group, AD model group, groups of AD rats administered with low, medium, and high doses of QKF, and the donepezil group. AD was established by bilateral injection of beta-amyloid (A beta) 1-40 into the hippocampus. Two days after AD was established, drugs were administered by gavage. After 14 days of treatment, we used RT-PCR, Western blotting, and immunohistochemistry to measure the transcript expression and protein abundance of A beta, IL-1 beta, and GFAP, and methenamine silver staining was used to detect amyloid protein particle deposition. Results. Compared to the control group, the rats from the AD model group showed significantly greater expression levels of A beta, IL-1 beta, and GFAP. However, these differences in expression were abolished by treatment with QKF or donepezil. Conclusion. QKF possesses therapeutic potential against AD because it downregulated A beta, IL-1 beta, and GFAP in the hippocampus of AD rats. Future studies should further examine the mechanisms through which QKF produces its effects and the consequences of long-term QKF administration.