The influence of enteral obestatin administration to suckling rats on intestinal contractility

摘要

This study investigated the effect of enteral administration of obestatin on the contractility of whole thickness preparations of duodenum and middle jejunum, as well as on the morphology of the enteric nervous system (ENS). Suckling rats were assigned to 3 groups (n = 12) treated with: C-saline solution; LO-obestatin (125 nmol/kg b.wt); HO-obestatin (250 nmol/kg b.wt). Saline solution or obestatin were administered twice daily, from the 14th to the 21st day of life. Sections were studied in an organ bath, for isometric recording in the presence of acetylocholine (ACh), atropine (ATR) and tetradotoxin (TTX). Thickness of intestinal muscularis layer, the number of interstitial cells of Cajal (ICC) were measured in the paraffin sections. The immunodetection of Muscarinic Acetylocholine Receptor 2 (M2 receptor) was performed in the intestinal segments.