首页> 外文期刊>Experimental and therapeutic medicine >Cystatin C promotes cognitive dysfunction in rats with cerebral microbleeds by inhibiting the ERK/synapsin Ia/Ib pathway
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Cystatin C promotes cognitive dysfunction in rats with cerebral microbleeds by inhibiting the ERK/synapsin Ia/Ib pathway

机译:胱抑素C通过抑制ERK / Symapsin IA / IB途径促进脑微微型大鼠的认知功能障碍

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摘要

Although higher serum level of cystatin C (CysC) was observed in patients with cerebral microbleeds, its associated role in the disease has not been elucidated. In this work, a rat model of cerebral microbleeds was created with the aim of investigating effects of CysC on cognitive function in rats with cerebral microbleeds and the underlying mechanism. Serum samples of patients with cerebral microbleeds and healthy people of the same age were collected. Levels of cystatin C expression in these samples were measured using CysC kits. Moreover, 48 spontaneously hypertensive rats (SHRs) bred under specific pathogen-free (SPF) conditions were randomly divided into 4 groups: sham surgery control group (sham), model group (CMB), model + empty vector control group (CMB + vehicle), and model + cystatin C overexpression group (CMB + CysC). Expression levels of CysC in hippocampus of rats in each group were measured by western blot analysis. The Y-maze was used to evaluate cognitive function of rats. Hippocampal long-term potentiation (LTP) in rats was assessed by the electrophysiological assay. Alterations in levels of p-ERK1/2 and p-synapsin Ia/b proteins associated with cognitive function were identified by western blot analysis. The serum levels of CysC in patients with cerebral microbleeds were significantly upregulated (P<0.001). After injection of CysC, its expression levels in rat hippocampus were significantly increased (P<0.001), which enhanced the decline in learning and memory function, as well as the decrease of LTP in the rat model of cerebral microbleeds (P<0.001). Western blot results showed that injection of CysC further reduced the levels of p-ERK1/2 and p-synapsin Ia/b in the rat model of microbleeds (P<0.001). CysC was up regulated in serum of patients with cerebral microbleeds. It promoted cognitive dysfunction in rats with microbleeds by inhibiting ERK/synapsin Ia/Ib pathway.
机译:虽然在脑微微患者患者中观察到更高的血清胱抑素C(CYSC),但其在疾病中的相关作用尚未阐明。在这项工作中,通过研究CySC对脑显微化和潜在机制的大鼠的认知功能的研究来创建大鼠脑微微脑微微的大鼠模型。收集脑微微患者血清样本和同一年龄的健康人。使用CySC试剂盒测量这些样品中的胱抑素C表达水平。此外,在特异性病原体(SPF)条件下,48种自发性高血压大鼠(SHR)随机分为4组:假手术对照组(假),模型组(CMB),型号+空向量对照组(CMB +车辆)和模型+胱抑素C过表达组(CMB + Cysc)。通过Western印迹分析测量每组大鼠大鼠海马中CySC的表达水平。 Y型迷宫用于评估大鼠的认知功能。通过电生理学测定评估大鼠中的海马长期增强(LTP)。通过Western印迹分析鉴定了与认知函数相关的p-ERK1 / 2和p-Synapsin Ia / B蛋白水平的改变。脑显微化患者血清CySC水平显着上调(P <0.001)。注射CYSC后,大鼠海马的表达水平显着增加(P <0.001),这提高了学习和记忆功能的下降,以及脑微比大鼠大鼠模型中LTP的降低(P <0.001)。 Western印迹结果表明,CysC的注射进一步降低了微杂化的大鼠模型中P-ERK1 / 2和P- Synapsin Ia / B的水平(P <0.001)。 Cysc在脑微微患者的血清中调节。通过抑制ERK / Synapsin Ia / Ib途径,它促进了用微比物的大鼠的认知功能障碍。

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  • 作者单位

    Qiqihar Med Univ Affiliated Hosp 3 Dept Phys Examinat Qiqihar 161000 Heilongjiang Peoples R;

    Qiqihar Med Univ Affiliated Hosp 3 Directors Off Qiqihar 161000 Heilongjiang Peoples R China;

    Qiqihar Med Univ Affiliated Hosp 3 Dept Neurol 1 27 Taishun St Qiqihar 161000 Heilongjiang;

    Qiqihar Med Univ Affiliated Hosp 3 Dept Neurol 1 27 Taishun St Qiqihar 161000 Heilongjiang;

    Qiqihar Med Univ Affiliated Hosp 3 Dept Neurol 3 Qiqihar 161000 Heilongjiang Peoples R China;

    Qiqihar Med Univ Affiliated Hosp 3 Dept Imagine Qiqihar 161000 Heilongjiang Peoples R China;

    Qiqihar Med Univ Affiliated Hosp 3 Dept Neurol 6 Qiqihar 161000 Heilongjiang Peoples R China;

    Qiqihar Med Univ Affiliated Hosp 3 Dept Neurol 1 27 Taishun St Qiqihar 161000 Heilongjiang;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 治疗学;
  • 关键词

    cystatin C; brain microbleeds; cognitive function;

    机译:胱抑素C;脑微微的;认知功能;

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