Long noncoding RNA CRNDE as a potential biomarker for the development and prognosis of cervical cancer

摘要

Objective: The long noncoding RNA CRNDE has been reported to be a good biomarker for poor prognosis in a variety of human cancers. However, whether CRNDE could serve as novel biomarker to predict prognosis in cervical cancer or not is unknown. The aim of the present study was to examine the expression of CRNDE in cervical cancers and to investigate the relationship between this lncRNA expression and cervical squamous cell cancer susceptibility and prognosis. Materials and Methods: The expression of CRNDE in 192 cervical cancer tissues and matched 192 adjacent normal tissues were detected by quantitative real-time RT-PCR and its correlation with the clinical characteristic and prognosis were analyzed. Results: The results showed that CRNDE was upregulated in 192 cervical cancer tissues compared with those in the adjacent normal tissues (p 0.001). Furthermore, CRNDE expression was positively correlated with FIGO stage (p 0.0001), lymph node metastasis (p 0.0001), depth of cervical invasion (p 0.0001), and tumor size (p = 0.006), but not other clinical characteristics. Moreover, Kaplan-Meier analysis demonstrated that decreased CRNDE expression contributed to poor overall survival (p 0.001) and disease-free survival (p 0.001) of patients. A multivariate survival analysis also indicated that CRNDE could be an independent prognostic marker. Furthermore, knockdown of CRNDE expression by small interfering RNA (siRNA) could inhibite cell proliferation and prompted cell apoptosis. Conclusions: The present data indicate that high expression of CRNDE is involved in cervical cancer progression and could he a potential target for diagnosis and gene therapy.