The acute effects of walking exercise intensity on systemic cytokines and oxidative stress

摘要

Purpose Oxidative stress is associated with tissue cytokine secretion although the precise mechanism(s) underpinning this relationship during high intensity intermittent exercise remains unclear. This study investigates the acute response to a bout of high intensity intermittent walking (HIIW), compared to continuous moderate intensity walking (CMW), on various cytokines and biomarkers of oxidative stress. Methods Seventeen ( n ?=?17) apparently healthy male participants (aged 22.6?±?4.6?years; $$dot{V}{ext{O}}_{2} max$$ V ˙ O 2 max : 53.7?±?7.1?ml?kg_(?1)?min_(?1)) undertook a randomised crossover study consisting of two exercise trials: (1) HIIW requiring 3?×?5?min bursts at 80% $$dot{V}{ext{O}}_{2} max$$ V ˙ O 2 max (each separated by 5?min of walking at 30% $$dot{V}{ext{O}}_{2} max$$ V ˙ O 2 max ) and (2) CMW (60% $$dot{V}{ext{O}}_{2} max$$ V ˙ O 2 max for 30?min). Each trial was separated by 7?days. Venous blood samples were obtained pre-exercise, post-exercise and at 2, 4, 24 and 48?h post-exercise for determination of systemic inflammation (IL-6 and TNF-α), lipid soluble antioxidants and oxidative stress (LOOH, H~(2)O~(2)and the ascorbyl free radical). Results Both IL-6 and TNF-α increased immediately post exercise, regardless of intensity and remained elevated until at least 4?h (main effect for time; p ?