A Novel Superoxide Anion Biosensor for Monitoring Reactive Species of Oxygen Released by Cancer Cells

Crulhas Bruno P.; Recco Lucas C.; Delella Flávia K.; Pedrosa Valber A.

摘要

Abstract > In this study, novel sensitive and selective hydrogel microstructures to detect superoxide anions released by cancer cells, based on electrochemical biosensors, are proposed. Ferrocene was coupled with superoxide dismutase within a poly(ethylene glycol) diacrylate hydrogel matrix. The pre‐polymer solution was patterned by photolithography in gold microelectrodes fabricated on top of glass slides. The biosensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry, and was able to detect superoxide anions in a wide linear range from 5?to 100 μM, with a low detection limit of 0.001?μM and sensitivity of 14.1 nA μM/mm 2 . Moreover, the biosensor was able to directly detect reactive oxygen species released from prostate cells. Furthermore, the reproducibility, stability and selectivity of the biosensor achieved better results when compared with the previous report, so this methodology can be used in physiological and pathological detection of reactive oxygen species, providing a powerful platform for clinical diagnostics in the future. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >提出了基于电化学生物传感器检测癌细胞释放的超氧化物阴离子的研究，新的敏感和选择性水凝胶微结构。二茂铁与聚（乙二醇）二丙烯酸酯水凝胶基质中的超氧化物歧化酶偶联。通过在玻璃载玻片顶部制造的金微电极中的光刻图案化预聚合物溶液。生物传感器的特征在于电化学阻抗光谱和循环伏安法，并且能够检测从5Ω宽的线性范围内的超氧化物阴离子，低检测限为0.001Ωμm，灵敏度为14.1μm/ mm <sup > 2 </ sup>。此外，生物传感器能够直接检测从前列腺细胞释放的反应性氧物质。此外，与先前的报告相比，生物传感器的再现性，稳定性和选择性达到了更好的结果，因此该方法可用于反应性氧物种的生理和病理检测，为未来提供强大的临床诊断平台。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2017年第5期</span><b style="margin: 0 2px;">|</b><span>共6页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Crulhas Bruno P.&option=202" target="_blank" rel="nofollow">Crulhas Bruno P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Recco Lucas C.&option=202" target="_blank" rel="nofollow">Recco Lucas C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Delella Flávia K.&option=202" target="_blank" rel="nofollow">Delella Flávia K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pedrosa Valber A.&option=202" target="_blank" rel="nofollow">Pedrosa Valber A.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Departament of Chemistry and BiochemistryInstitute of Bioscience UNESP-Botucatu SPBrazil;</p> <p>Departament of Chemistry and BiochemistryInstitute of Bioscience UNESP-Botucatu SPBrazil;</p> <p>Departament of MorphologyInstitute of Bioscience UNESP-Botucatu SPBrazil;</p> <p>Departament of Chemistry and BiochemistryInstitute of Bioscience UNESP-Botucatu SPBrazil;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174607.html">A Novel Superoxide Anion Biosensor for Monitoring Reactive Species of Oxygen Released by Cancer Cells</a> <b>[J]</b> . <span> <a 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href="/conference-cn-11493/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十一届全国电分析化学会议 </a> <span> <span> . 2011</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020312254238.html">新型场效应晶体管生物传感器用于心衰相关标志物和中药调控心肌细胞钙离子释放的监测</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=雷用敏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 雷用敏</a> <span> . 2017</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120113138920.html">用于药物释放监测的邻二硫醇反应性治疗探针及制备</a> <b>[P]</b> . <span> 中国专利： CN113173952A </span> <span> . 2021-07-27</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120113196723.html">基于量子点的生物传感器制备及癌细胞摄取监测方法</a> <b>[P]</b> . <span> 中国专利： CN113237855A </span> <span> . 2021-08-10</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130499859451.html">Compound, composition including the same, and method of monitoring reactive oxygen species superoxide radical anion</a> <b>[P]</b> . <span> 外国专利：  KR20210042750A </span> <span> . 2021-04-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：化合物，组合物，包括该组合物，以及监测反应性氧物质超氧化物的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130500621724.html">Compound composition including the same and method of monitoring reactive oxygen species superoxide radical anion</a> <b>[P]</b> . <span> 外国专利：  KR102302260B1 </span> <span> . 2021-09-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：包括相同和监测反应性氧物质超氧化物根部的相同和方法的复合组合物 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130422981972.html">METHODS OF REPRODUCING THE CONCENTRATION OF REACTIVE OXYGEN SPECIES IN THE PROSTATE OF A HUMAN HUMAN BEING, PROPHILICALLY TREATING A PROSTATE HUMAN HUMAN HUMAN CANCER POLYAMINE OXIDASE IN THE PROSTATE OF A HUMAN HUMAN BEING, DETERMINING OXIDATIVE STRESS IN HUMAN TISSUE, TREATING CANCER PROSTATE, REPRODUCING THE CONCENTRATION OF OXYGEN DEXYENE AND KIN DEXTENE SPECIES OXIDATIVE FABRIC STRESS OF MALE HUMAN PROSTATE, ORAL PHARMACEUTICAL COMPOSITION, KIT AND REAGENTS TO MEASURE CONCENTRATION OF EX VIVO OR IN VIVO REACTIVE OXYGEN SPECIES, ORGAN OR ORGAN CELL</a> <b>[P]</b> . <span> 外国专利：  BRPI0711280A2 </span> <span> . 2012-03-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在人的前列腺中复制活性氧的浓度的方法，在人的前列腺中对人的前列腺癌多胺氧化酶进行特效处理，确定在人的治疗中的氧化应激，治疗的过程氧，二甲苯种的浓度人体前列腺的氧化性纤维应激，口腔药物成分，试剂盒和试剂测定活体内或活体内活性氧物种，器官或器官细胞的浓度 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023174607','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div 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