Conjugated Molecule Based Sensor for Microbial Detection in Water with        <i >E. coliE. coli        as a Case Study and Elucidation of Interaction Mechanism

Mallya Ashwini N.; Ramamurthy Praveen C.

摘要

Abstract > Water testing for microbial contamination is essential to ensure safe drinking water. In the present work, an organic nanocomposite based sensor is designed and fabricated to detect the presence of E. coli in water. The detection is carried out by measuring the change in two parameters of the organic nanocomposite film – resistance and impedance. The lower detection limit of E. coli cell counts up to 10?CFU?mL ?1 and 10 7 ?CFU?mL ?1 in 100?mL test solution is observed by impedance spectroscopy and resistance change respectively. A conjugated molecule with a specific pendant amine group was used as the receptor moiety that can interact and exhibit affinity to the functional groups like carboxyl groups present on the outer membrane of the cell wall of the E. coli . The binding of E. coli cells to conjugated molecule was characterized by chemical, physical and structural properties. The electrostatic interaction between positively charged amine groups on conjugated molecule and negatively charged E. coli is utilized for sensing. This interaction is also observed to be acting as p‐dopant to conjugated molecule which synergistically induces a change in electrical resistance in the composite. This work shows that the conjugated molecules designed with suitable moieties could be used for sensing microorganisms, when most sensors for detection of E. coli cells, use antibodies as detecting element. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >微生物污染的水测试对于确保安全饮用水至关重要。在本作工作中，设计和制造基于有机纳米复合材料的传感器以检测 e的存在。 Coli </ i>在水中。通过测量有机纳米复合膜抗性和阻抗的两个参数的变化来进行检测。较低的检测限 e。 Coli </ i>电池最多10？cfu？ml α1</ sup>和10 7 </ sup>Δcfu？ml α1</ sup>在100？ml中通过阻抗光谱和电阻变化观察测试溶液。用特定侧胺基组的共轭分子用作容受体部分，其可以与存在于<I> E的细胞壁的外膜上存在的羧基相互作用并表现出与羧基的亲和力。 Coli </ i>。 e的结合。 Coli </ i>对共轭分子的细胞的特征在于化学，物理和结构性。缀合分子上带正电荷胺基团之间的静电相互作用，带负电荷 e。 Coli </ i>用于感测。还观察到该相互作用是用作缀合分子的p掺杂剂，其协同诱导复合材料中的电阻变化。这项工作表明，当大多数传感器检测<i的传感器时，可以用于感测微生物的共轭分子。 COLI </ i>细胞，使用抗体作为检测元件。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第6期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mallya Ashwini N.&option=202" target="_blank" rel="nofollow">Mallya Ashwini N.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ramamurthy Praveen C.&option=202" target="_blank" rel="nofollow">Ramamurthy Praveen C.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Materials EngineeringIndian Institute of ScienceBangalore- 560 012 India;</p> <p>Department of Materials EngineeringIndian Institute of ScienceBangalore- 560 012 India;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sensor&option=203" rel="nofollow">Sensor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=amine receptor&option=203" rel="nofollow">amine receptor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=conjugated molecule&option=203" rel="nofollow">conjugated molecule;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chemiresistor&option=203" rel="nofollow">chemiresistor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=impedance&option=203" rel="nofollow">impedance;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=E. coli&option=203" rel="nofollow">E. coli;</a> </p> <div class="translation"> 机译：传感器;矿物受体;共轭分子;杂交电阻;阻抗;e。大肠杆菌; 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COLI F18 INTESTINE COLONIZATION OR INTESTINE DISORDERS RELATED TO E. COLI F18, ISOLATED DNA MOLECULES, A POLYPEPTIDE, A POLYNUCLEOTIDE, MOLECULAR DETERMINATION FOR THE DETECTION OF THE E. COLI F18 RECEPTORS, A KIT FOR THE DETECTION OF THE E. COLI F18 RECEPTORS AND USE OF MUTANT FORM OF THE ALPHA-1,2-FUCOSYL TRANSFERASE 1 GENE OF PIG</a> <b>[P]</b> . <span> 外国专利：  PL195890B1 </span> <span> . 2007-11-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：鉴定具有抗E.COLI F18肠道菌落或与E.COLI F18相关的肠道疾病的免疫力的猪的方法，分离的DNA分子，多肽，多核苷酸，分子测定法以检测大肠杆菌。猪大肠杆菌F18受体检测试剂盒和猪ALPHA-1,2-岩藻糖基转移酶1基因突变形式的使用 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130422589450.html">A protein fragment complementation assay (PCA) for the detection of Protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. Coli Tem-1-B-lactamase</a> <b>[P]</b> . <span> 外国专利：  AU2012203377A1 </span> <span> . 2012-07-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于大肠杆菌Tem-1-B-内酰胺酶的蛋白质片段互补分析（PCA），用于检测蛋白质-蛋白质，蛋白质-小分子和蛋白质核酸相互作用 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130422643306.html">A protein fragment complementation assay (PCA) for the detection of Protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. Coli Tem-1-B-lactamase</a> <b>[P]</b> . <span> 外国专利：  AU2008201746B2 </span> <span> . 2012-03-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于大肠杆菌Tem-1-B-内酰胺酶的蛋白质片段互补分析（PCA），用于检测蛋白质-蛋白质，蛋白质-小分子和蛋白质核酸相互作用 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023174504','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img 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Conjugated Molecule Based Sensor for Microbial Detection in Water with E. coliE. coli as a Case Study and Elucidation of Interaction Mechanism

摘要