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Specific and Rapid Glucose Detection Using NAD‐dependent Glucose Dehydrogenase, Diaphorase, and Osmium Complex

机译：使用NAD依赖性葡萄糖脱氢酶，透明酶和锇络合物进行特异性和快速的葡萄糖检测

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Abstract > Selective glucose measurement in serum and blood and rapid glucose measurement using nicotinamide adenine dinucleotide (NAD)‐dependent glucose dehydrogenase (NAD‐GDH) are still very challenging. Here, we report a selective and rapid glucose sensor, based on electrochemical‐enzymatic‐enzymatic (ENN) redox cycling involving bis(2,2‐bipyridyl)dichloroosmium(II) [Os(bpy) <sub>2</sub> Cl <sub>2</sub> ], diaphorase (DI), NAD + , NAD‐GDH, and glucose. DI and Os(bpy) <sub>2</sub> Cl <sub>2</sub> are used to obtain fast mediated oxidation of NADH that is generated as a result of glucose oxidation by NAD‐GDH. DI and NAD‐GDH are co‐immobilized via affinity binding on an avidin‐modified indium tin oxide electrode to obtain fast and stable ENN redox cycling. Two enzymes (DI and NAD‐GDH) and two electron mediators [Os(bpy) <sub>2</sub> Cl <sub>2</sub> and NAD + ] are insensitive to oxygen. The applied potential (0.0?V?vs Ag/AgCl) is low enough to minimize interfering electrochemical reactions, and the redox reactions of Os(bpy) <sub>2</sub> Cl <sub>2</sub> with interfering species are slow. NAD‐GDH is much less reactive to problematic monosaccharides such as xylose, fructose, galactose, and mannose than glucose. Artificial serum containing 5?% (w/v) human serum albumin shows a similar electrochemical background level in serum. All results enable us to obtain selective and reproducible glucose detection. The fast ENN redox cycling allows sensitive glucose detection with a wide range of concentrations in artificial serum with a short measuring time (5?s) without an incubation period. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract type =“main”xml：lang =“en”> <标题类型=“main”>摘要</ title> >使用烟草酰胺腺嘌呤二核苷酸（nad）的血清和血液和快速血糖测量的选择性葡萄糖测量 - 依赖性葡萄糖脱氢酶（NAD-GDH）仍然非常具有挑战性。在此，我们报告一种基于电化学 - 酶 - 酶（ENN）氧化还原循环的选择性和快速的葡萄糖传感器，涉及双（2,2-双吡啶基）二氯铵（II）[OS（BPY）<Sub> 2 </ sub> Cl <sub> 2 </ sub>，透明酶（di），nad + </ sup>，nad-gdh和葡萄糖。 DI和OS（BPY）<sub> 2 </ sub> Cl <sub> 2 </ sub>用于获得由于NAD-GDH的葡萄糖氧化而产生的NADH的快速介导的氧化。 DI和NAD-GDH通过抗生物素蛋白改性的氧化铟锡电极上的亲和结合共同固定，以获得快速且稳定的烯烃氧化还原循环。两种酶（DI和NAD-GDH）和两种电子介质[OS（BPY）<sub> 2 </ sub> CL <sub> 2 </ sub>和NAD + </ sup>对氧气不敏感。施加的电位（0.0≤V≤Ag/ AgCl）足够低，以最小化干扰电化学反应，以及OS（BPY）<sub> 2 </ sub> Cl <sub> 2 </ sub>的氧化还原反应与干扰物种很慢。 NAD-GDH对有问题的单糖（如木糖，果糖，半乳糖和甘露糖）的反应性远低得多。含有5μl的人造血清（W / V）人血清白蛋白在血清中显示出类似的电化学背景水平。所有结果使我们能够获得选择性和可重复的葡萄糖检测。快速enn氧化还原循环允许敏感葡萄糖检测在人造血清中具有宽范围的浓度，其中测量时间短（5？s），没有孵育期。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haque Al‐Monsur Jiaul&option=202" target="_blank" rel="nofollow">Haque Al‐Monsur Jiaul;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nandhakumar Ponnusamy&option=202" target="_blank" rel="nofollow">Nandhakumar Ponnusamy;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Haesik&option=202" target="_blank" rel="nofollow">Yang Haesik;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemistry and Chemistry Institute for Functional MaterialsPusan National UniversityBusan 46241 Korea;</p> <p>Department of Chemistry and Chemistry Institute for Functional MaterialsPusan National UniversityBusan 46241 Korea;</p> <p>Department of Chemistry and Chemistry Institute for Functional MaterialsPusan National UniversityBusan 46241 Korea;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Glucose&option=203" rel="nofollow">Glucose;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Glucose dehydrogenase&option=203" rel="nofollow">Glucose dehydrogenase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Diaphorase&option=203" rel="nofollow">Diaphorase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Osmium complex&option=203" rel="nofollow">Osmium complex;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Redox cycling&option=203" rel="nofollow">Redox cycling;</a> </p> <div class="translation"> 机译：葡萄糖;葡萄糖脱氢酶;透明酶;锇络合物;氧化还原循环; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174369.html">Specific and Rapid Glucose Detection Using NAD‐dependent Glucose Dehydrogenase, Diaphorase, and Osmium Complex</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haque Al‐Monsur Jiaul&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Haque Al‐Monsur Jiaul,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nandhakumar Ponnusamy&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nandhakumar Ponnusamy,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Haesik&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Haesik </a> <a href="/journal-foreign-22546/" target="_blank" rel="nofollow" class="tuijian_authcolor">Electroanalysis .</a> <span>2019</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用NAD依赖性葡萄糖脱氢酶，透明酶和锇络合物进行特异性和快速的葡萄糖检测</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070404782472.html">Detection and purification of glucose 6-phosphate dehydrogenase, malic enzyme, and NADP-dependent isocitrate dehydrogenase by blue native polyacrylamide gel electrophoresis</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Beriault R&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Beriault R,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chenier D&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chenier D,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Singh R&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Singh R,</a> <a href="/journal-foreign-22425/" target="_blank" rel="nofollow" class="tuijian_authcolor">Electrophoresis: The Official Journal of the International Electrophoresis Society .</a> <span>2005</span><span>,第15期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：蓝天然聚丙烯酰胺凝胶电泳检测和纯化葡萄糖6-磷酸脱氢酶，苹果酸酶和NADP依赖性异柠檬酸脱氢酶</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204119116238.html">Effect of adrenaline and phorbol myristate acetate or bacterial lipopolysaccharide on stimulation of pathways of macrophage glucose, glutamine and O2 metabolism. Evidence for cyclic AMP-dependent protein kinase mediated inhibition of glucose-6-phosphate dehydrogenase and activation of NADP+-dependent ‘malic’ enzyme</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C Murphy&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">C Murphy,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=L F B P Costa Rosa&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">L F B P Costa Rosa,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=P Newsholme&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">P Newsholme,</a> <a href="/journal-foreign-30556/" target="_blank" rel="nofollow" class="tuijian_authcolor">The biochemical journal .</a> <span>1995</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：肾上腺素和佛波醇肉豆蔻酸酯或细菌脂多糖对巨噬细胞葡萄糖，谷氨酰胺和O2代谢途径的刺激作用。环状AMP依赖性蛋白激酶介导的抑制6磷酸葡萄糖脱氢酶和激活NADP +依赖性“苹果酸”酶的证据</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-293807_thesis/0705018960441.html">Glucose Sensor Using NAD-Dependent Glucose Dehydrogenase, Diaphorase, and Osmium Complex</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=SangWook Nam&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">SangWook Nam,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haesik Yang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Haesik Yang </a> <a href="/conference-foreign-293807/" target="_blank" rel="nofollow" class="tuijian_authcolor">Meeting of the Electrochemical Society;International Meeting on Chemical Sensors .</a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用NAD依赖性葡萄糖脱氢酶，透明酶和锇络合物的葡萄糖传感器</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061204822.html">The glucose and insulin responsiveness of the rat glucose-6-phosphate dehydrogenase promoter.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Arkwright-Keeler, Daryl.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Arkwright-Keeler, Daryl. </a> <span>2005</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：大鼠葡萄糖6-磷酸脱氢酶启动子的葡萄糖和胰岛素反应性。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040003487507.html">Effect of adrenaline and phorbol myristate acetate or bacterial lipopolysaccharide on stimulation of pathways of macrophage glucose glutamine and O2 metabolism. Evidence for cyclic AMP-dependent protein kinase mediated inhibition of glucose-6-phosphate dehydrogenase and activation of NADP+-dependent malic enzyme.</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=L F Costa Rosa&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">L F Costa Rosa,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=R Curi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">R Curi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C Murphy&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">C Murphy,</a> <span>1995</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：肾上腺素和佛波肉豆蔻酸酯乙酸盐或细菌脂多糖对巨噬细胞葡萄糖谷氨酰胺和O2代谢途径的刺激作用。环状AMP依赖性蛋白激酶介导的葡萄糖-6-磷酸脱氢酶抑制和NADP +依赖性苹果酸酶活化的证据。</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100037930778.html ">Effect of adrenaline and phorbol myristate acetate or bacterial lipopolysaccharide on stimulation of pathways of macrophage glucose, glutamine and O2 metabolism. Evidence for cyclic AMP-dependent protein kinase mediated inhibition of glucose-6-phosphate dehydrogenase and activation of NADP+-dependent 'malic' enzyme.</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Costa Rosa, L F&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Costa Rosa, L F,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Curi, R&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Curi, R,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Murphy, C&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Murphy, C,</a> <span>1995</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：肾上腺素和佛波肉豆蔻酸酯乙酸盐或细菌脂多糖对巨噬细胞葡萄糖，谷氨酰胺和O2代谢途径的刺激作用。环状AMP依赖性蛋白激酶介导的葡萄糖-6-磷酸脱氢酶抑制和NADP +依赖性“苹果酸”酶活化的证据。</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_pb_thesis/020711452754.html">Development and Evaluation of a Hexokinase/Glucose-6-Phosphate Dehydrogenase Procedure for Use as a National Glucose Reference Method,</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=neese,jane w. duncan,patricia &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">neese,jane w. duncan,patricia </a> <span>1978</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：己糖激酶/葡萄糖-6-磷酸脱氢酶程序的开发和评价用作国家葡萄糖参考方法，</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-medical-research_thesis/0201285911277.html">红细胞6-磷酸葡萄糖脱氢酶单次洗涤快速动力学分析新方法</a> <b>[J]</b> <span> <a 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