Genome-wide identification and prediction of long non-coding RNAs in half-smooth tongue sole Cynoglossus semilaevis

摘要

At present, the function and profile of long noncoding RNAs (IncRNAs) in half-smooth tongue soles Cynoglossus semilaevis remain poorly understood. Lherefore, we identified IncRNAs in the species using large-scale deep sequencing approaches. A total of 96 726 222 clean reads and 2497 IncRNAs were obtained from the compound samples. In total, 1 694 known IncRNAs and 803 novel IncRNAs were identified. Most of the novel IncRNAs distributed mainly in a range of 200-3 000 nt and contained 2-6 exons. Six novel IncRNAs were selected for expression analysis by quantitative reverse transcription-polymerase chain reaction, of which lnc_770 and lnc_150 were expressed mainly in the ovary. Lhis study provides a valuable resource for IncRNA studies of half-smooth tongue sole and improves our understanding of the function of non-coding RNA in fish.