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首页> 外文期刊>International Journal of Genomics >The Numerical Predominance and Large Transcriptome Differences of Neutrophils in Peripheral Blood Together Inevitably Account for a Reported Pulmonary Tuberculosis Signature
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The Numerical Predominance and Large Transcriptome Differences of Neutrophils in Peripheral Blood Together Inevitably Account for a Reported Pulmonary Tuberculosis Signature

机译:外周血中嗜中性粒细胞的数值优势和大转录组差异不可避免地占据了报告的肺结核签名

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摘要

Previous transcriptomic analysis revealed a 393-transcript signature (PTBsig), which is dominated by interferon inducible genes, in whole blood of pulmonary tuberculosis (PTB) patients. Comparisons with a limited set of interferon-driven genes among separated monocytes, CD4+ T cells, CD8+ T cells, and neutrophils indicated that the signature is due to changes in neutrophils, the overwhelmingly predominant cell type. By extending the analysis to the entire 393 transcripts of PTBsig and by switching the cell proportions between separated monocytes, CD4+ T cells, CD8+ T cells, and neutrophils, we create putative PTBsig for whole blood (pPTBsig) in which CD4+ or CD8+ T cells or monocytes predominated or in which the cell proportions were unchanged. These putative signatures are then compared to the actual reported PTBsig. We show that, because of their predominance in peripheral blood and their larger transcriptional responses, neutrophils were indeed almost exclusively responsible for PTBsig. We caution that the functional significance of changes in other cell types might escape notice in transcriptome analysis that is based upon whole blood.
机译:以前的转录组分析显示了一种393-转录物签名(PTBSIG),其由干扰素诱导基因为主,在肺结核(PTB)患者的全血中。分离的单核细胞,CD4 + T细胞,CD8 + T细胞和中性粒细胞中具有有限的干扰素驱动基因的比较表明签名是由于中性粒细胞的变化,绝对的主要细胞类型。通过将分析扩展到PTBSIG的整个393转录物,并通过在分离的单核细胞,CD4 + T细胞,CD8 + T细胞和中性粒细胞之间切换细胞比例,为全血(PPTBSIG)产生推定的PTBSIG,其中CD4 +或CD8 + T细胞或单核细胞主要的或其中细胞比例不变。然后将这些推定的签名与实际报告的PTBSIG进行比较。我们表明,由于它们在外周血中的优势和较大的转录反应,中性粒细胞确实只关注PTBSIG。我们注意,其他细胞类型的变化的功能意义可能会在基于全血的转录组分析中逃逸通知。

著录项

  • 来源
    《International Journal of Genomics》 |2017年第1期|共8页
  • 作者单位

    Shanghai Public Health Clinical Center Key Laboratory of Medical Molecular Virology of MOE/MOH Fudan University 2901 Caolang Road Shanghai 201508 China;

    Shanghai Medical College Fudan University 138 Yixueyuan Road Shanghai 200032 China;

    StateKey Laboratory of Medical Genomics Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200025 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
  • 关键词

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