Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-gamma in peripheral blood mononuclear cells (PBMCs)

摘要

Recombinant horse cytokines may be used as biological adjuvant for enhancing vaccine efficacy and therapeutic agent. This study describes low cost production of recombinant horse IL-2, IL-18 and IFN-gamma in prokaryotic system. lmmunomodulatory activities of recombinant horse IL-2, IL-18 and IFN-gamma have been demonstrated in horse peripheral blood mononuclear cells (PBMCs). Recombinant horse IL-2 and IL-18 enhanced cellular proliferation and significantly up regulated transcription and secretion of IFN-gamma, TNF-alpha, IL-4, IL-10 and IL-12p35. In contrast, recombinant horse IFN-gamma was comparatively less active and did not show significant immune-stimulatory effect.