Evaluation of function of RICTOR gene in gga-miR-142-3p knockdowned developing chicken embryo

摘要

MicroRNAs (miRNAs) are a large classes of endogenous non coding RNAs of about 18-21 nucleotides long. It regulates the gene expression post-transcriptionally by binding partial or complete complementary sites in 3' UTRs. The miR-142-3p was highly expressed in all native cell lineages of haematopoietic organs. It has been reported that miR-142-3p was highly expressed in embryonic day 15 and day 20 in spleen and bursa of Fabricius of chicken. The present study was planned to knock down the gga-miR142-3p in chicken embryonic developmental stages for identification of its role in morphogenesis at embryonic spleen and bursa of Fabricius development. Knock down of miR-142-3p in the 14 days old embryonic eggs was carried out by intravenous inoculation of LNA modified miR-142-3p inhibitor. Organs were harvested from the embryos at the embryonic day of 20 and analyzed for gross pathological, histopathological changes and RICTOR gene expressions have been analyzed using SYBR Green qPCR technology. The results indicated that the RICTOR gene was upregulated in all the immune organs and visceral organs except kidney. The over expression of RICTOR gene lead to defective actin reorganization in the cells that will affect the change in the cell structural integrity and cell survival. Over expression of RICTOR gene is associated with increased proliferation of cells along with invasion of tumor cells.