Anti‐inflammatory and antioxidant properties of Schisandrin C promote mitochondrial biogenesis in human dental pulp cells

摘要

Abstract Aim To examine the properties of Schisandrin C as an anti‐inflammatory and antioxidant compound, and whether its characteristics promote mitochondrial biogenesis in human dental pulp cells ( HDPC s). Methodology HDPC s were extracted from fresh third molars and cultured for experiments. Reactive oxidative stress ( ROS ) and nitric oxide ( NO ) formation were analysed by a Muse cell analyser. Western blotting and gelatin zymography were used to identify the presence of antioxidants, as well as anti‐inflammatory and mitochondrial biogenesis with specific antibody. An unpaired Student's t ‐test was used for statistical analysis. Results Schisandrin C inhibited lipopolysaccharide‐stimulated inflammatory molecules; interleukin 1 beta, tumour necrosis factor alpha, intracellular adhesion molecule‐1, vascular cell adhesion molecule‐1, matrix metalloproteinase‐2 and ‐9, NO production, ROS formation, nuclear factor kappa B translocation ( P? ? 0.05) through the mitogen‐activated protein kinase pathway. Schisandrin C increased the expression of superoxide dismutase enzymes as well as haem oxygenase‐1 and peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha through the phosphorylated‐protein kinase B (p‐Akt) and nuclear factor erythroid 2‐related factor‐2 pathways ( P? ? 0.05). The anti‐inflammatory and antioxidant properties of Schisandrin C promoted mitochondrial biogenesis. Conclusions Schisandrin C has the potential to reduce inflammation and oxidation and to promote mitochondrial biogenesis. Therefore, Schisandrin C may be considered for use as an anti‐inflammatory compound for oral inflammation through mitochondrial biogenesis.