Comparison of the new Mie Extinction Extended Multiplicative Scattering Correction and Resonant Mie Extended Multiplicative Scattering Correction in transmission infrared tissue image scattering correction

摘要

The correction of optical effects in infrared spectroscopy has been developing over the years with Resonant Mie Extended Multiplicative Scattering Correction marking a significant point. A new open-source algorithm of Mie Extinction Extended Multiplicative Scattering Correction has been released in 2019, giving a much needed flexibility for the user. The new approach uses a Van der Hulst approximation for absorbing spheres instead of full Mie theory, significantly speeding up the calculations. In this article a comparison of both algorithms is done in the context of biological tissue imaging with a Focal Plane Array, which has its own characteristics over single animal cell measurements or IR confocal measurements.