Plasmodium infection in Kerteszia cruzii (Diptera: Culicidae) in the Atlantic tropical rain forest, southeastern Brazil

摘要

In Southeastern Brazil, Kerteszia cruzii (former Anopheles cruzii), a bromeliad mosquito species, is considered an efficient human Plasmodium spp. vector. In this region, recent studies showed asymptomatic or sub-patent Plasmodium falciparum infection. In areas of the Atlantic coast in Rio de Janeiro, Plasmodium simium infection was recently reported in both human and howler monkey. Considering that (1) few malaria cases are reported each year in areas across the tropical Atlantic rain forest in southeastern Brazil; (2) malaria elimination in Atlantic forest is challenged by circulation of P. falciparum and P. simium in humans; (3) the complexity of malaria epidemiology in this region; and (4) the public health importance of Kerteszia cruzii as a sylvatic vector; the major goal of this study is to evaluate Plasmodium infection in Ke. cruzii. Mosquito sampling collections were conducted in Esteiro do Morro and Sitio Itapuan, in Cananeia municipality, and Tapirai municipality in Ribeira Valley, southeastern Sao Paulo state, Brazil. Influence of climate and landscape factors in Plasmodium infection in Ke. cruzii was addressed. Among the 1719 mosquitoes tested, 3 females collected in Sitio Itapuan and three from Tapirai were found infected with either P. vivax or P. simium. Results of statistical analyses did not demonstrate association between Plasmodium infection in mosquito and the landscape. Mosquito infection was found in two landscape clusters, with Plasmodium detected in forest fringe mosquitoes. This finding shows that Ke. cruzii can facilitate transmission among human and non-human primates. Plasmodium falciparum was not identified in the samples analyzed. Spatiotemporal variation in local malaria incidence, low prevalence of Plasmodium, variations in humidity and temperature can explain the absence of mosquitoes infected with P. falciparum in the study.