EVALUATION OF HEPATO-PROTECTIVE ACTIVITY OF K-LIV DS ON CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN RATS

摘要

Present study investigates K-Liv DS, a polyherbal formulation on carbon tetrachloride induced hepatotoxicity in Wistar rats. Rats were divided into normal control, CCI_4 control, CCI_4+K-Liv DS (0.5 mL) and CCI_4+K-LivDS (1 mL). CCI_4 was injected intraperitoneal from day 1 to day 10, while K Liv DS was administered orally for 14 days. Hepatoprotective activity was assessed by estimating transaminases, alkaline phosphatase, total protein and bilirubin in serum. Estimation of oxidative stress markers and histopathological study of liver were also carried out. Metabolic function of liver was evaluated by thiopentone induced sleeping time. CCI_4 administration produced a significant hepatotoxicity (P<0.001), which was reversed with K-Liv DS treatment. It significantly decreased transaminases, alkaline phosphatase and bilirubin and increased total proteins (P<0.001). Moreover oxidative stress was decreased and metabolic function was improved by decrease in sleeping time. Histopathological examination revealed no abnormalities in the K-Liv DS treated rats. K-Liv DS is an effective hepatoprotective agent and has potential clinical applications for liver diseases.