Susceptibility and comparative bioassay study for determination of fluconazole concentrations in Candida spp. by broth macrodilution and disc diffusion methods

摘要

Fluconazole (FLZ) is a broad range antifungal agent clinically used to treat mycoses. Only few methods are available for quantifications of FLZ in biological samples. HPLC, though the method of choice, is expensive and laborious. Here, we proposed an economical simple method [broth macrodilution (BMD) and disc diffusion (DDT)] for quantification of FLZ. A comparative study for susceptibility and bioassay testing of fluconazole was conducted with six Candida isolates by broth macrodilution and disc diffusion method. Both the methods were performed according to the National Committee for Clinical Laboratory Standards (NCCLS) recommendations (M-27A). The endpoint of macrodilution (BMD) method and disc diffusion (DDT) method were minimum inhibitory concentration (MIC90) and zone of inhibition after 48 and 72 h of incubation, respectively. The susceptibility and bioassay performance including the minimum inhibitory concentration, MIC90, and linearity coefficient (r(2)) were found satisfactory.