Identification of Phytochemical Constituents In the Leaf Extracts of Azima tetracantha Lam using Gas Chromatography-Mass Spectrometry(GC-MS)analysis and Antioxidant Activity

摘要

Azima tetracantha LAM.is widely used in traditional medicine for treatment of cancer and diabetic activity.Both its leaves were reported to possess antioxidant,antibacterial and anti-inflammatory activities.The leaves of Azima tetracantha LAM.were extracted with petroleum ether,chloroform,ethyl acetate and ethanol by cold maceration extraction procedure and the resultant extract was subjected to phytochemical analyses using GC-MS technique.Three compounds with known biological activities were detected and identified based on GC-MS data as Friedelin,Isorhamnetin-3-o-rufmoside,Myricetin.The findings of the study support the usage of Azima tetracantha LAM in traditional medicinal as most of the identified compounds are known to possess biological activities.The present study was designed to investigate the anti-oxidant activity of the ethyl acetate and ethanolic extract of Azima tetracantha LAM.The ethyl acetate and ethanolic extract was evaluated by using 1,1-Diphenyl-2-Picrylhydrazyl(DPPH),hydrogen peroxide and xanthine oxidase inhibition assay.The ethyl acetate and ethanolic extract of Azima tetracantha LAM exhibited highest level of antioxidant activities.The ethyl acetate and ethanolic extract of Azima tetracantha LAM showed up to 66% and 67% inhibition of DPPH free radical at 517 nm at high concentration of 1 mg/ml.The in-vitro xanthine oxidase inhibition of ethyl acetate and ethanolic extract revealed significant 68% and 69% inhibitory activity at 30u.g/ml and it compared with the standard drug quercetin which showed 70% inhibition at 30μg/ml.In hydrogen peroxide scavenging activity the ethyl acetate and ethanolic extract shows 26.55% and 27.45% of inhibition when compared to the standard ascorbic acid.The presence of strong antioxidant activity of ethyl acetate and ethanolic extract of Azima tetracantha LAM can be a great scope for further studies on this plant regarding its antioxidant potentials.