Polyphasic taxonomic characterisation of a novel strain as Pararhizobium haloflavum sp. nov., isolated from soil samples near a sewage treatment tank

摘要

Abstract A Gram-stain negative, aerobic, motile and ovoid- to rod-shaped bacteria strain, designated XC0140_(T), was isolated from soil samples near the sewage treatment tank of a chemical factory in Zhejiang Province, China, and subjected to polyphasic taxonomic investigation. Strain XC0140_(T)grew at 10–37?°C and pH 6.0–9.0 (optimum, 35?°C and pH 7.5) and with 0–17% (w/v) NaCl (optimum, 1%). According to phylogenetic analysis based on 16S rRNA gene sequences, strain XC0140_(T)was assigned to the genus Pararhizobium with high 16S rRNA gene sequence similarity of 95.97% to “ Pararhizobium helanshanense CCNWQTX14_(T”), followed by Pararhizobium sphaerophysae CCNWGS0238_(T)(95.95%). Chemotaxonomic analysis showed that strain XC0140_(T)contains ubiquinone-10 as the predominant respiratory quinone and possessed summed feature 8 (comprising C~(18: 1)ω7c and/or ω6c), 11-methyl C~(18:1)ω7c, C~(18: 0)and C~(16: 0)as predominant forms of fatty acids. The polar lipids of strain XC0140_(T)consisted of seven phospholipids (PL), two aminolipids (AL), one glycolipid (GL) and three unidentified lipids (L1, L2 and L3). The DNA G+C content was 62.7?mol%. Based on the polyphasic taxonomic characterization, strain XC0140_(T)is considered to represent a novel species of the genus Pararhizobium , for which the name Pararhizobium haloflavum sp. nov. is proposed. (type strain XC0140_(T)?=?MCCC 1K03228_(T)?=?KCTC 52582_(T)).