UV/VIS spectrophotometric methods for determination of caffeine and phenylephrine hydrochloride in complex pharmaceutical preparations. Validation of the methods.

摘要

The contents of active substances were determined in a preparation TP-4 (tablets) containing paracetamol, ascorbic acid, caffeine and phenylephrine hydrochloride. For determination of caffeine and phenylephrine hydrochloride, UV/VIS spectrophotometric method was used. The VIS spectrophotometric method based on the reaction of phenylephrine with ninhydrine in sulphuric acid (1.127 kg/l). Validation of methods performed for model mixtures proved those methods were accurate, precise, repeatable and linear in the range from 50% to 150% of the amount declared in the preparation. The content of caffeine and phenylephrine hydrochloride in TP-4, Thompyrin, Panadol Extra, Ring N satisfies the FP V demands.