Effects of Level, Loading Rate, Injury and Repair on Biomechanical Response of Ovine Cervical Intervertebral Discs

摘要

A need exists for pre-clinical large animal models of the spine to translate biomaterials capable of repairing intervertebral disc (IVD) defects. This study characterized the effects of cervical spinal level, loading rate, injury and repair with genipin-crosslinked fibrin (FibGen) on axial and torsional mechanics in an ovine cervical spine model. Cervical IVDs C2–C7 from nine animals were tested with cyclic tension–compression (??240 to 100?N) and cyclic torsion (±?2° and?±?4°) tests at three rates (0.1, 1 and 2?Hz) in intact, injured and repaired conditions. Intact IVDs from upper cervical levels (C2–C4) had significantly higher torque range and torsional stiffness and significantly lower axial range of motion (ROM) and tensile compliance than IVDs from lower cervical levels (C5–C7). A tenfold increase in loading rate significantly increased torque range and torsional stiffness 4–8% (depending on amplitude) ( p ?