Sperm quality characteristics and fertilization capacity after cryopreservation of rooster semen in extender exposed to a magnetic field

摘要

There has not been as much success in the effectiveness of avian semen cryopreservation as that of mammalian semen cryopreservation. This has been attributed to the unique characteristics of poultry sperm. In the area of mammalian semen, the use of magnetized extenders has enhanced the cryo-resistance of sperm in some species. The applicability of this strategy to avian semen has not yet been evaluated. The purpose of this study was to evaluate the effects on sperm quality and fertility after magnetization of extenders and use of these extenders for cryopreservation of rooster semen. Extenders were passed between two neodymium magnets that produced different magnetic induction values (0, 2000, 4000 and 6000 G). Physicochemical properties of the extenders were assessed and then semen samples were cryopreserved in the magnetized extenders. The smallest cluster sizes and zeta potentials in the extenders were observed in the extender magnetized at 2000 gauss (G). There were greater percentages of sperm total motility, viability and mitochondrial membrane potential in semen treated with the extender magnetized at 2000 G A greater percentage of sperm with enhanced membrane functionality was observed when sperm were stored in extenders magnetized with 2000 G and 4000 G. In a field trial, there were greater percentages of fertility and hatching rates of eggs using semen treated with extenders magnetized at 2000 G and 4000 G. Cryopreservation of rooster semen with magnetized extenders at 2000-4000 G resulted in enhanced quality indices of thawed sperm as well as the fertility capacity of the cryopreserved semen.