When to Choose FFF: Interview With Dr. Christoph Johann of Wyatt Technology

摘要

Field-flow fractionation (FFF) has slowly evolved since its introduction in 1976. Its principal advantage is the robust, size-based separation of protein aggregates, large polymers, and subvisible particles. In this regard, FFF complements size exclusion chromatography (SEC), which also separates molecules by size, but starts to lose resolving power as one moves up in the mega-dalton (MDa) range. While FFF can readily separate particles from 1 nm and up, the sweet spot for FFF is MDa to about one micrometer.