Sesamin Ameliorates Insulin Resistance Induced by High Glucose/High Insulin in Hepatic LO2 Cells

摘要

In this study, the antioxidant effect of sesamin on insulin resistance in hepatic L02 cells induced by high glucose/high insulin was investigated. Results showed that treatment with sesamin (200,100 ug/ml) increased the cell viability, activity of super-oxide dismutase and glutathione peroxidase, and the content of reduced glutathione of L02 hepatocyte model of insulin-resistance. Moreover, treatment with sesamin decreased the content of malondialdehyde, production of nitric oxide, and the activity of nitric oxide synthase, inducible nitric oxide synthase, and gene expression levels of c-Jun NH2-terminal kinase significantly. The destructive change of hepatic L02 cells was ameliorated when treated with sesamin under microscopic observation. In conclusion, sesamin has anti-oxidative effect on the L02 hepatocyte model of insulin resistance induced by high glucose/high insulin. Sesamin is a lignan (Fig. 1) with a molecular weight of 354.36. Sesamin has been shown to have multiple beneficial health effects including promotion of immune function, anti-oxidative effects, antitumor activity, amelioration of dyslipidemia, and protection of hepatocytes (Kami et al., 2007; Bian, 2008; Dong et al., 2007; Wei et al., 2008; Wang and Song, 2006; Nobuo et al., 2005). In an earlier study, our laboratory reported amelioration of insulin-resistance in KK-Ay mice, a model for type 2 diabetes (Lei et al., 2013b). The major focus of this study was to further explore the role and mechanism of the sesamin amelioration of insulin resistance. To this end, we have employed the L02 hepatocyte model of insulin resistance induced by high glucose and high insulin.